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Purification and Properties of an Exo-α-l,3-glucanase fromTrichoderma viride
| Content Provider | Scilit |
|---|---|
| Author | Tsunoda, Akira Nagaki, Tameichiro Sakano, Yoshiyuki Kobayashi, Tsuneo |
| Copyright Year | 1977 |
| Description | Journal: Bioscience, Biotechnology, and Biochemistry An α-l,3-glucanase was partially purified from Meicelase, a commercial cellulase preparation from T. viride, and its properties were studied. Cellulase in the crude enzyme was removed by adsorption on cellulose powder, and α-l,3-glucanase was purified through salting- out with ammonium sulfate, chromatographies on DEAE-Sephadex, hydroxylapatite and Sephadex G-100. The α-l,3-glucanase gave 2 peaks on DEAE-Sephadex chromatography, and the enzyme of the second peak was further purified to about 300-fold purification. The enzyme was most active at pH 4.5, and 55°C, stable at 4°C at pH between 3 and 7, and stable up to 50°C on heat treatment at pH 4.5 for 30 min. The Michaelis constant for α-l,3-glucan was 7.1 mM (as anhydroglucose units). The molecular weight of the enzyme determined by gel filtration on Sephadex G-100 was 47,000. Only glucose was formed as reducing sugar liberated from α-l,3-glucan, showing that the enzyme was an exo-α-l,3-glucanase (EC 3.2.1.84). |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/00021369.1977.10862611?needAccess=true |
| Ending Page | 943 |
| Page Count | 5 |
| Starting Page | 939 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/00021369.1977.10862611 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 6 |
| Volume Number | 41 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1977-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Paper and Wood Chromatographies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
