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Purification and Some Properties of Flint Corn α-Glucosidase
| Content Provider | Scilit |
|---|---|
| Author | Chiba, Seiya Shimomura, Tokuji |
| Copyright Year | 1975 |
| Description | An α-glucosidase was purified from flint corn by precipitation with ammonium sulfate, chromatographies on CM-cellulose and Hydroxylapatite and gel-filtrations on Sephadex G-100. The purified enzyme was homogeneous in ultracentrifugal and disc electrophoretic analysis. The sedimentation coefficient was calculated to be 6.5 S. The molecular weight was estimated to be approximately $6.5×10^{4}$ by gel-filtration technique. The optimal pH was found to be 3.6 for both maltose and soluble starch. The enzyme lost about 80% of the activity by incubation at 60°C for 10 min. The ratio of velocity of hydrolysis for maltose, phenyl-α-glucoside and soluble starch was estimated to be 100:14.3:6.1 in this order. The αglucosidase hydrolyzed soluble starch exo-wisely. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/00021369.1975.10861723?needAccess=true |
| Ending Page | 1040 |
| Page Count | 8 |
| Starting Page | 1033 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/00021369.1975.10861723 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 5 |
| Volume Number | 39 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1975-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Biochemical Research Corn Optimal Incubation Flint Lost Purified Chromatographies Α Glucosidase |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
