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Purification and Properties of β-Glucosidase fromHumicola insolensYH-8
| Content Provider | Scilit |
|---|---|
| Author | Yoshioka, Hajime Hayashida, Shinsaku |
| Copyright Year | 1980 |
| Description | The thermophilic fungus, Humicola insolens YH-8 exhibited high β-glucosidase activity when grown in solid wheat bran medium. The β-glucosidase was purified from the culture extract by consecutive column chromatographies and found to be homogeneous on polyacrylamide gel disc electrophoresis. The molecular weight was estimated to be 250,000 by SDS-gel electrophoresis, and the isoelectric point was at pH 4.23. The enzyme had an optimum pH of 5.0, an optimum temperature of 50°C, and showed significant resistance to urea, dimethyl sulfoxide and ethyl alcohol. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/00021369.1980.10864208?needAccess=true |
| Ending Page | 1735 |
| Page Count | 7 |
| Starting Page | 1729 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/00021369.1980.10864208 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 8 |
| Volume Number | 44 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1980-08-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Paper and Wood Mycology Alcohol Electrophoresis Gel Glucosidase Isoelectric Chromatographies Fromhumicola |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
