Loading...
Please wait, while we are loading the content...
Enzymatic Decolorization of Melanoidin byCoriolussp. No. 20
| Content Provider | Scilit |
|---|---|
| Author | Watanabe, Yoshio Sugi, Ryozo Tanaka, Yonemi Hayashida, Shinsaku |
| Copyright Year | 1982 |
| Description | Journal: Bioscience, Biotechnology, and Biochemistry Coriolus sp. No. 20 decolorized a melanoidin solution, a decrease of about 80% in darkness under the optimal conditions. This decolorization occurred with an intracellular enzyme which was prepared from an extract of integrated mycelia, and required aeration and some kinds of sugars, particularly glucose and sorbose. The fraction with melanoidin-decolorizing activity was collected and purified by DEAE-cellulose and Sephadex G-200 column chromatographies. The optimal pH and temperature were pH 4.5 and 35°C, respectively. The molecular weight was found to be about 200,000 by SDS-gel electrophoresis. The purified enzyme was identified as sorbose oxidase; decolorization proceeded in the presence of oxygen and sugars such as maltose, sucrose, lactose, galactose and xylose, besides glucose and sorbose. Glucose in the reaction mixture was converted to gluconic acid. Melanoidin was suggested to be decolorized by the active oxygen formed. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/00021369.1982.10865299?needAccess=true |
| Ending Page | 1630 |
| Page Count | 8 |
| Starting Page | 1623 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/00021369.1982.10865299 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 6 |
| Volume Number | 46 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1982-06-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Paper and Wood Electrophoresis Chromatographies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
