NDLI: Large-Scale Fluorescence Imaging in Neuroscience

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Large-Scale Fluorescence Imaging in Neuroscience

Content Provider	Scilit
Author	Prevedel, Robert
Copyright Year	2020
Description	This chapter discusses the underlying principles of multiphoton microscopy (MPM) and the technical realization. A major challenge in the field of neuroscience is to understand how the orchestrated activity of millions of neurons leads to cognitive function and behavior. The chapter is devoted to some more exciting and upcoming techniques in MPM, especially regarding imaging deep in living tissues and improving the imaging speed and capacity to distinguish multiple fluorophores. Most multiphoton imaging experiments that deal with live and scattering tissue will likely be limited at some point by the attainable image depth or at least will face rapidly decreasing signal and contrast when trying to image deeper. Being able to image multiple fluorophores or “colors” simultaneously is becoming increasingly important in biology for a variety of purposes, such as visualizing different specific molecules in a subcellular context, recording activity and signaling events, but also for tracking cell anatomy, movement, and lineage. Book Name: Imaging from Cells to Animals In Vivo
Related Links	https://content.taylorfrancis.com/books/download?dac=C2016-0-43082-9&isbn=9781315174662&doi=10.1201/9781315174662-25&format=pdf
Ending Page	348
Page Count	12
Starting Page	337
DOI	10.1201/9781315174662-25
Language	English
Publisher	Informa UK Limited
Publisher Date	2020-12-03
Access Restriction	Open
Subject Keyword	Book Name: Imaging From Cells To Animals In Vivo Biomedical Engineering Behavior Function Tissues Neuroscience Image Mpm Multiphoton Chapter Multiple Fluorophores
Content Type	Text
Resource Type	Chapter

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