Loading...
Please wait, while we are loading the content...
Similar Documents
Live Imaging of Zebrafish
| Content Provider | Scilit |
|---|---|
| Author | Wan, Yinan Keller, Philipp J. Höckendorf, Burkhard |
| Copyright Year | 2020 |
| Description | This chapter reviews some fluorescent labeling strategies that are commonly used in zebrafish in the order of decreasing sparseness, focusing on their respective strengths and weaknesses for live imaging. From the making of a protein molecule to the division of a cell, from the generation of an action potential to the motion of an animal, all biological processes intrinsically have a dynamic nature. The chapter discusses the general principles and challenges in the design of light-based live imaging methods, and then introduces several microscopy techniques that are commonly used and actively researched for zebrafish studies. Although the inhomogeneous properties of zebrafish tissues can provide a certain level of optical contrast in label-free embryos, modern microscopy relies heavily on fluorescent labeling techniques and has benefited tremendously from the development of fluorescent dyes and proteins. Ubiquitous labeling is suitable for in toto imaging of the entire zebrafish embryo or specific tissues at early developmental stages. Book Name: Imaging from Cells to Animals In Vivo |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2016-0-43082-9&isbn=9781315174662&doi=10.1201/9781315174662-23&format=pdf |
| Ending Page | 324 |
| Page Count | 18 |
| Starting Page | 307 |
| DOI | 10.1201/9781315174662-23 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-12-03 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Imaging From Cells To Animals In Vivo Biomedical Engineering Embryos Proteins Tissues Zebrafish Live Imaging Fluorescent Labeling |
| Content Type | Text |
| Resource Type | Chapter |
