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Cutting Edge: Transcriptional Profiling Reveals Multifunctional and Cytotoxic Antiviral Responses of Zika Virus–Specific CD8+ T Cells
| Content Provider | Scilit |
|---|---|
| Author | Grifoni, Alba Costa-Ramos, Priscilla Pham, John Tian, Yuan Rosales, Sandy L. Seumois, Grégory Sidney, John de Silva, Aruna D. Premkumar, Lakshmanane Collins, Matthew H. Stone, Mars Norris, Phillip J. Romero, Claudia M. E. Durbin, Anna Ricciardi, Michael J. Ledgerwood, Julie E. de Silva, Aravinda M. Busch, Michael Peters, Bjoern Vijayanand, Pandurangan Harris, Eva Falconar, Andrew K. Kallas, Esper Weiskopf, Daniela Sette, Alessandro |
| Copyright Year | 2018 |
| Description | Zika virus (ZIKV) constitutes an increasing public health problem. Previous studies have shown that $CD8^{+}$ T cells play an important role in ZIKV-specific protective immunity. We have previously defined antigenic targets of the ZIKV-specific $CD8^{+}$ T cell response in humans. In this study, we characterized the quality and phenotypes of these responses by a combined use of flow cytometry and transcriptomic methods, using PBMCs from donors deriving from different geographical locations collected in the convalescent phase of infection. We show that ZIKV-specific $CD8^{+}$ T cells are characterized by a polyfunctional IFN-γ signature with upregulation of TNF-α, TNF receptors, and related activation markers, such as CD69, as well as a cytotoxic signature characterized by strong upregulation of GZMB and CRTAM. The signature is stable and not influenced by previous dengue virus exposure, geographical location, or time of sample collection postinfection. To our knowledge, this work elucidates the first in-depth characterization of human $CD8^{+}$ T cells responding to ZIKV infection. |
| Ending Page | 3491 |
| Page Count | 5 |
| Starting Page | 3487 |
| ISSN | 00221767 |
| e-ISSN | 15506606 |
| DOI | 10.4049/jimmunol.1801090 |
| Journal | The Journal of Immunology |
| Issue Number | 12 |
| Volume Number | 201 |
| Language | English |
| Publisher | The American Association of Immunologists |
| Publisher Date | 2018-12-15 |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
| Subject | Immunology and Allergy Immunology |
