NDLI: In vitro DNA adduct profiling to mechanistically link red meat consumption to colon cancer promotion

Please wait, while we are loading the content...

An in vivo study in mice: mother's gestational exposure to organophosphorus pesticide retards the division and migration process of neural progenitors in the fetal developing brain

Mussel-inspired PEGylated carbon nanotubes: biocompatibility evaluation and drug delivery applications

Modifications of H3K9me2, H3K36me3 and H4K20me2 may be involved in arsenic-induced genetic damage

Development of predictive QSAR models for Vibrio fischeri toxicity of ionic liquids and their true external and experimental validation tests

Epigenetic disruption and glucose homeostasis changes following low-dose maternal bisphenol A exposure

Investigations of the genotoxic properties of two synthetic cathinones (3-MMC, 4-MEC) which are used as psychoactive drugs

Oxidative stress and inflammatory responses involved in dietary nickel chloride (NiCl₂)-induced pulmonary toxicity in broiler chickens

Proparacaine induces cytotoxicity and mitochondria-dependent apoptosis in corneal stromal cells both in vitro and in vivo

Lipopolysaccharide attached to urban particulate matter 10 suppresses immune responses in splenocytes while particulate matter itself activates NF-κB

Silica nanoparticles induce start inhibition of meiosis and cell cycle arrest via down-regulating meiotic relevant factors

Lead aggravates the diabetic-induced reproductive toxicity in male Wistar rats

Fluoride-induced headkidney macrophage cell apoptosis involves activation of the CaMKIIg-ERK 1/2-caspase-8 axis: the role of superoxide in initiating the apoptotic cascade

Back cover

Toxicology Research Vol - 5 Issue - 4

Toxicology Research Vol - 5 Issue - 3

Toxicology Research Vol - 5 Issue - 2

Toxicology Research Vol - 5 Issue - 1

Toxicology Research Vol -4

Toxicology Research Vol -3

Toxicology Research Vol -2

Toxicology Research Vol -1

In vitro DNA adduct profiling to mechanistically link red meat consumption to colon cancer promotion

Content Provider	Royal Society of Chemistry (RSC)
Author	Hecke, Thomas Van Hemeryck, Lieselot Y. Rombouts, Caroline Vanhaecke, Lynn Meulebroek, Lieven Van Smet, Stefaan De Bussche, Julie Vanden
Copyright Year	2016
Abstract	Colorectal cancer (CRC) is the third most common cancer type in the world. Epidemiological research has demonstrated that both red and processed meat consumption significantly contribute to CRC risk. In this study, red meat toxicity was investigated by means of simulated gastrointestinal conditions, malondialdehyde (MDA) analysis and UHPLC-(HR)MS(/MS) based DNA adductomics. Since dairy products with high calcium content are associated with a decreased CRC-risk, the possible CRC-protective effects of calcium were assessed as well. The obtained results confirmed the earlier reported finding that heme-rich meat stimulates lipid peroxidation and O6-carboxymethylguanine (O6-CMG) DNA adduct formation during digestion. Calcium carbonate (CaCO3) supplementation resulted in both toxic and anti-toxic effects; i.e. stimulation of O6-CMG production, but reduction of MDA formation. DNA adductome mapping of meat digests revealed a significant interindividual variability. The observed DNA adduct profile also differed according to the digested meat type, uncovering different putative DNA adducts that seem to be associated with digestion of beef or chicken with or without supplemented CaCO3. Formamidopyrimidine-adenine was found to be discriminative for meat digests without added CaCO3, carboxyethylcytosine was significantly higher in beef digests and methoxymethylcytosine (or its hydroxyethylcytosine isomer) was found to be lower in meat digests supplemented with CaCO3. These results demonstrate that DNA adduct formation may be involved in the pathway that links red meat digestion to CRC promotion. In addition, the possible CRC-protective attributes of calcium through anti-oxidant actions could be documented.
Starting Page	1346
Ending Page	1358
Page Count	13
File Format	HTM / HTML PDF
ISSN	2045452X
Volume Number	5
Issue Number	5
Journal	Toxicology Research
DOI	10.1039/c6tx00079g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Calcium Colorectal cancer Order of St Michael and St George Lipid peroxidation DNA adduct Red meat MDA Malondialdehyde Isomer CRC DNA Calcium carbonate Chicken
Content Type	Text
Resource Type	Article
Subject	Health, Toxicology and Mutagenesis Toxicology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Calcium carbonate suppresses haem toxicity markers without calcium phosphate side effects on colon carcinogenesis.

Heme-induced biomarkers associated with red meat promotion of colon cancer are not modulated by the intake of nitrite.

Red Meat-Derived Nitroso Compounds, Lipid Peroxidation Products and Colorectal Cancer

Red Meat and Colorectal Cancer.

Roles of Lipid Peroxidation-Derived Electrophiles in Pathogenesis of Colonic Inflammation and Colon Cancer

Calcium carbonate suppresses haem toxicity markers without calcium phosphate side effects on colon carcinogenesis

Oxidative DNA damage and formalin-fixation procedures

Meat Consumption and Risk of Colorectal Cancer

Determining Oxidative Damage by Lipid Peroxidation Assay in Rat Serum.

In vitro DNA adduct profiling to mechanistically link red meat consumption to colon cancer promotion

Similar Documents

Calcium carbonate suppresses haem toxicity markers without calcium phosphate side effects on colon carcinogenesis.

Heme-induced biomarkers associated with red meat promotion of colon cancer are not modulated by the intake of nitrite.

Red Meat-Derived Nitroso Compounds, Lipid Peroxidation Products and Colorectal Cancer

Red Meat and Colorectal Cancer.

Roles of Lipid Peroxidation-Derived Electrophiles in Pathogenesis of Colonic Inflammation and Colon Cancer

Calcium carbonate suppresses haem toxicity markers without calcium phosphate side effects on colon carcinogenesis

Oxidative DNA damage and formalin-fixation procedures

Meat Consumption and Risk of Colorectal Cancer

Determining Oxidative Damage by Lipid Peroxidation Assay in Rat Serum.

In vitro DNA adduct profiling to mechanistically link red meat consumption to colon cancer promotion