NDLI: High resolution magic angle spinning NMR investigation of silk protein structure within major ampullate glands of orb weaving spiders

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Soft Matter Vol - 8 Issue - 5

High resolution magic angle spinning NMR investigation of silk protein structure within major ampullate glands of orb weaving spiders

Content Provider	Royal Society of Chemistry (RSC)
Author	Yarger, Jeffery L. Jenkins, Janelle E. Holland, Gregory P.
Copyright Year	2012
Abstract	Spider silks display some of the most impressive mechanical properties of known fibrous materials. However, a molecular level understanding of how spiders store and convert the highly concentrated protein-rich fluid in the gland to an insoluble super fiber at the spinneret remains unanswered. In this report, we demonstrate the utility of High-Resolution Magic Angle Spinning (HR-MAS) NMR spectroscopy to study the molecular structure of the proteins within excised major ampullate (MA) glands from two orb weaving spiders, Nephila clavipes and Argiope aurantia. The spiders were fed various 13C-enriched amino acids to target different regions of the proteins and enable multi-dimensional, multinuclear HR-MAS NMR experiments. Comparisons are made between these HR-MAS results on MA glands and previous solid-state NMR data on spider dragline silk. The conformation-dependent 13C and 1H isotropic chemical shifts obtained in this study indicate that both dragline silk proteins, major ampullate spidroin 1 (MaSp1) and 2 (MaSp2), are present in a random coil conformation in the MA gland prior to fiber formation for both spider species.
Starting Page	1947
Ending Page	1954
Page Count	8
File Format	HTM / HTML PDF
ISSN	1744683X
Volume Number	8
Issue Number	6
Journal	Soft Matter
DOI	10.1039/c2sm06462f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spinneret (polymers) Solid-state nuclear magnetic resonance Dragline excavator Golden silk orb-weaver Nuclear magnetic resonance spectroscopy Master's degree Telephassa Isotropy MASP2 (protein) Spider silk MASP1 (protein) Spider Nuclear magnetic resonance Random coil Protein structure
Content Type	Text
Resource Type	Article
Subject	Chemistry Condensed Matter Physics

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