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| Content Provider | Royal Society of Chemistry (RSC) |
|---|---|
| Author | Demirkol, Dilek Odaci Guler, Cetin Timur, Suna Yalcinkaya, Esra Evrim Ag, Didem Seleci, Muharrem |
| Copyright Year | 2012 |
| Abstract | Clay based biosensors were developed using montmorillonite (Mont) modified with methyl (M) and dimethylamine (DM). X-ray diffraction, Fourier transform infrared spectroscopy, zeta potential and thermal gravimetric measurements were used to characterize the modified clays. After immobilization of glucose oxidase (GOx) via clay on the glassy carbon electrode, its application as a glucose biosensor was investigated in detail. The best response characteristics were obtained by DM-Mont and optimization of enzyme amount, reproducibility of biosensor fabrication, repeatability of measurements and operational stability were all evaluated. The optimized biosensor showed a very good linearity between 0.05 mM and 1.0 mM, a 7 s response time and a limit of detection to glucose of 0.038 mM. Also, kinetic parameters and stabilities were determined. Apparent Km and Imax values were found as 0.73 mM and 2.955 μA, respectively. As well as batch configuration, the DM-Mont/GOx biosensor was successfully applied in the flow injection analysis mode. Finally, the performance of the DM-Mont/GOx biosensor to analyze glucose in a wine sample was compared with HPLC. |
| Starting Page | 2112 |
| Ending Page | 2118 |
| Page Count | 7 |
| File Format | HTM / HTML PDF |
| ISSN | 20462069 |
| Volume Number | 2 |
| Issue Number | 5 |
| Journal | RSC Advances |
| DOI | 10.1039/c2ra01225a |
| Language | English |
| Publisher | Royal Society of Chemistry |
| Access Restriction | Open |
| Subject Keyword | Wine Glucose oxidase Electrode Flow injection analysis Montmorillonite Enzyme Fourier-transform infrared spectroscopy Glucose High-performance liquid chromatography Carbon X-ray crystallography Fourier Zeta potential Biosensor Dimethylamine Clay minerals IMAX Mathematical optimization |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Chemical Engineering |
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