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Development of an efficient immobilization matrix based on a conducting polymer and functionalized multiwall carbon nanotubes: synthesis and its application to ethanol biosensors

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Journal of Materials Chemistry B Vol -1

Development of an efficient immobilization matrix based on a conducting polymer and functionalized multiwall carbon nanotubes: synthesis and its application to ethanol biosensors

Content Provider	Royal Society of Chemistry (RSC)
Author	Kanik, Fulya Ekiz Hacioglu, Serife O. Uzun, Sema Demirci Soylemez, Saniye Toppare, Levent
Copyright Year	2014
Abstract	Material modification is one of the hot topics recently. Hereby a novel functional monomer, 2-(4-nitrophenyl)-4,7-di(thiophen-2-yl)-1H-benzo[d]imidazole (BIPN), was synthesized for matrix generation through electrochemical polymerization. Its conducting polymer was successfully used for the biolayer construction in the biosensor preparation. The electrochemical and morphological properties were improved by the introduction of carboxylic acid functionalized multiwall carbon nanotubes (f-MWCNTs). Carboxylic acid functionalization of MWCNTs was carried out via acid treatment. The electrode surface was modified with the polymer and f-MWCNTs during electropolymerization to achieve a perfect immobilization matrix for alcohol oxidase. In order to prepare a new alcohol biosensor, alcohol oxidase (AOx) was immobilized onto the modified electrode. The modified electrode was characterized by scanning electron microscopy (SEM), X-ray photoelectron microscopy (XPS) and Fourier transform infrared (FTIR) spectroscopy techniques. Electrochemical responses of the enzyme electrodes were monitored at −0.7 V vs. Ag reference electrode by monitoring oxygen consumption in the presence of ethanol. Kinetic parameters, operational and storage stabilities were investigated. KappM, Imax, LOD and sensitivity were calculated as 16.946 mM, 3.31 μA, 0.806 mM and 476 μA mM−1 cm−2, respectively. Finally, this biosensor was applied to estimate the alcohol content in various beverages successfully.
Starting Page	511
Ending Page	521
Page Count	11
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	2
Issue Number	5
Journal	Journal of Materials Chemistry B
DOI	10.1039/c3tb21356k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Fourier Monomer Imidazole Composite material Electrochemistry Polymerization Conductive polymer Biosensor Carboxylic acid Carbon nanotube Electrode Polymer Nanoarchitectures for lithium-ion batteries Oxidase Scanning electron microscope X-ray crystallography Photoemission spectroscopy Fourier transform Infrared spectroscopy Spectroscopy Enzyme Reference electrode Oxygen Ethanol IMAX
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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