NDLI: Magnetic CoPt nanoparticles as MRI contrast agent for transplanted neural stem cells detection

Content Provider	Royal Society of Chemistry (RSC)
Author	Thanh, Nguyen T. K. Lu, Le T. Seton, Hugh C. Prior, Ian A. Meng, Xiaoting Song, Bing
Copyright Year	2011
Abstract	Neural stem cells (NSCs) exhibit features that make them suitable candidates for stem cell replacement therapy and spinal cord reconstruction. Magnetic resonance imaging (MRI) offers the potential to track cellsin vivo using innovative approaches to cell labeling and image acquisition. In this study, experiments were carried out to optimize the loading condition of magnetic CoPt hollow nanoparticles (CoPt NPs) into neural stem cells and to define appropriate MRI parameters. Both cell viability and multipotency analysis showed that CoPt NPs at a concentration of 16 µg ml−1 reduced T2 relaxation times in labeled rat NSCs, producing greater contrast on spin echo acquisitions at 4.7 T, yet did not affect cell viability and in vitro differentiation potential compared to controls. After optimizing nanoparticle loading concentrations and labeled cell numbers for MRI detection, CoPt-loaded NSCs were transplanted into organotypic spinal cord slices. The results showed that MRI could efficiently detect low numbers of CoPt-labeled NSCs with the enhanced image contrast. Our study demonstrated that MRI of grafted NSCs labeled with CoPt NPs is a useful tool to evaluate organotypic spinal cord slice models and has potential applications in other biological systems.
Starting Page	977
Ending Page	984
Page Count	8
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	3
Issue Number	3
Journal	Nanoscale
DOI	10.1039/c0nr00846j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	NSCs Spinal cord Magnetic resonance imaging Nanoparticle Stem cell Spin echo
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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