NDLI: Highly efficient colorimetric detection of target cancer cells utilizing superior catalytic activity of graphene oxide

Content Provider	Royal Society of Chemistry (RSC)
Author	Kim, Moon Il Kang, Kyoung Suk Lee, Jinwoo Park, Hyun Gyu Woo, Min-Ah Ye, Youngjin Kim, Min Su
Copyright Year	2014
Abstract	Enzyme-linked immunosorbent assays (ELISAs) have most widely been applied in immunoassays for several decades. However, several unavoidable limitations (e.g., instability caused by structural unfolding) of natural enzymes have hindered their widespread applications. Here, we describe a new nanohybrid consisting of Fe3O4 magnetic nanoparticles (MNPs) and platinum nanoparticles (Pt NPs), simultaneously immobilized on the surface of graphene oxide (GO). By synergistically integrating highly catalytically active Pt NPs and MNPs on GO whose frameworks possess high substrate affinity, the nanohybrid is able to achieve up to a 30-fold higher maximal reaction velocity (Vmax) compared to that of free GO for the colorimetric reaction of the peroxidase substrate, 3,3′,5,5′-tetramethylbenzidine (TMB), and enable rapid detection of target cancer cells. Specifically, using this new assay system, clinically important breast cancer cells are detected in a 5 min time period at room temperature with high specificity and sensitivity. The remarkably high capability to catalyze oxidation reactions could allow the nanohybrid to replace conventional peroxidase-based immunoassay systems as part of new, rapid, robust and convenient assay systems which can be widely utilized for the identification of important target molecules.
Starting Page	1529
Ending Page	1536
Page Count	8
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	6
Issue Number	3
Journal	Nanoscale
DOI	10.1039/c3nr05539f
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Immunoassay Graphene Vmax TMB Peroxidase Breast cancer Cancer
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

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