NDLI: A Novel Colorimetric Immunoassay Utilizing the Peroxidase Mimicking Activity of Magnetic Nanoparticles (2013)

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A Novel Colorimetric Immunoassay Utilizing the Peroxidase Mimicking Activity of Magnetic Nanoparticles (2013)

Content Provider	CiteSeerX
Author	Park, Hyun Gyu
Abstract	Abstract: A simple colorimetric immunoassay system, based on the peroxidase mimicking activity of Fe3O4 magnetic nanoparticles (MNPs), has been developed to detect clinically important antigenic molecules. MNPs with ca. 10 nm in diameter were synthesized and conjugated with specific antibodies against target molecules, such as rotaviruses and breast cancer cells. Conjugation of the MNPs with antibodies (MNP-Abs) enabled specific recognition of the corresponding target antigenic molecules through the generation of color signals arising from the colorimetric reaction between the selected peroxidase substrate, 3,3',5,5'-tetramethylbenzidine (TMB) and H2O2. Based on the MNP-promoted colorimetric reaction, the target molecules were detected and quantified by measuring absorbance intensities corresponding to the oxidized form of TMB. Owing to the higher stabilities and economic feasibilities of MNPs as compared to horseradish peroxidase (HRP), the new colorimetric system employing MNP-Abs has the potential of serving as a potent immunoassay that should substitute for conventional HRP-based immunoassays. The strategy employed to develop the new methodology has the potential of being extended to the construction of simple diagnostic systems for a variety of biomolecules related to human cancers and infectious diseases, particularly in the realm of point-of-care applications.
File Format	PDF
Publisher Date	2013-01-01
Access Restriction	Open
Subject Keyword	Peroxidase Substrate Human Cancer Mnp-promoted Colorimetric Reaction Infectious Disease Economic Feasibility Specific Recognition Corresponding Target Antigenic Molecule Point-of-care Application Absorbance Intensity Potent Immunoassay Fe3o4 Magnetic Nanoparticles New Methodology Breast Cancer Cell New Colorimetric System Oxidized Form Magnetic Nanoparticles Simple Colorimetric Immunoassay System Conventional Hrp-based Immunoassay Important Antigenic Molecule Colorimetric Reaction Specific Antibody Color Signal Simple Diagnostic System Target Molecule Peroxidase Mimicking Activity
Content Type	Text
Resource Type	Article

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