NDLI: A quartz nanopillar hemocytometer for high-yield separation and counting of CD4<sup>+</sup> T lymphocytes

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A quartz nanopillar hemocytometer for high-yield separation and counting of CD4⁺ T lymphocytes

Back matter

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Nanoscale Vol - 4 Issue - 6

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Nanoscale Vol - 4 Issue - 4

Nanoscale Vol - 4 Issue - 3

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Nanoscale Vol -1

A quartz nanopillar hemocytometer for high-yield separation and counting of CD4⁺ T lymphocytes

Content Provider	Royal Society of Chemistry (RSC)
Author	Kim, Dong-Joo Lee, Seung-Yong Wu, Yu Seol, Jin-Kyeong Fan, Rong Ji, Seungmuk Kim, Gil-Sung Lim, Hyuneui Lee, Sang-Kwon Hyung, Jung-Hwan
Copyright Year	2012
Abstract	We report the development of a novel quartz nanopillar (QNP) array cell separation system capable of selectively capturing and isolating a single cell population including primary CD4+ T lymphocytes from the whole pool of splenocytes. Integrated with a photolithographically patterned hemocytometer structure, the streptavidin (STR)-functionalized-QNP (STR-QNP) arrays allow for direct quantitation of captured cells using high content imaging. This technology exhibits an excellent separation yield (efficiency) of ∼95.3 ± 1.1% for the CD4+ T lymphocytes from the mouse splenocyte suspensions and good linear response for quantitating captured CD4+ T-lymphoblasts, which is comparable to flow cytometry and outperforms any non-nanostructured surface capture techniques, i.e. cell panning. This nanopillar hemocytometer represents a simple, yet efficient cell capture and counting technology and may find immediate applications for diagnosis and immune monitoring in the point-of-care setting.
Starting Page	2500
Ending Page	2507
Page Count	8
File Format	HTM / HTML PDF
ISSN	20403364
Volume Number	4
Issue Number	7
Journal	Nanoscale
DOI	10.1039/c2nr11338d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Hemocytometer Flow cytometry Nanopillar CD4 Streptavidin Splenocyte Quartz Computer mouse T helper cell
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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A quartz nanopillar hemocytometer for high-yield separation and counting of CD4⁺ T lymphocytes

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