NDLI: Construction of biosensor systems for determining the pathophysiological potential of carrageenan variants

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Construction of biosensor systems for determining the pathophysiological potential of carrageenan variants

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Molecular BioSystems Vol - 5 Issue - 8

Molecular BioSystems Vol - 5 Issue - 7

Molecular BioSystems Vol - 5 Issue - 6

Molecular BioSystems Vol - 5 Issue - 5

Molecular BioSystems Vol - 5 Issue - 4

Molecular BioSystems Vol - 5 Issue - 3

Molecular BioSystems Vol - 5 Issue - 2

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Construction of biosensor systems for determining the pathophysiological potential of carrageenan variants

Content Provider	Royal Society of Chemistry (RSC)
Author	Riedel, Angela Renner, Marcus Bender, Christian Schmidt, Steffen Sueltmann, Holger End, Caroline Christiansen, Helle Mollenhauer, Jan Korn, Bernhard
Copyright Year	2009
Abstract	In vitro systems for monitoring safety of nutritional additives are desirable for high-throughput screenings and as a substitute for animal models. Carrageenan (CGN) is a sulfated polysaccharide widely used as a thickener and texturizer in human nutrition and is intensely discussed regarding its pathophysiological potential. Low molecular weight (lm) variants of CGN are considered to exert more profound pathophysiological effects in vivo than high molecular weight (hm) variants. We used a systematic approach to construct reporter systems allowing distinction between CGN-variants with different pathophysiological potential. Reporter systems utilizing segments of the CGN-responsive DMBT1 promoter did not display substantial activity in SW620 cells of intestinal epithelial origin. Genome-wide profiling revealed stronger qualitative and quantitative changes in global gene activities for hm-CGN than for lm-CGN (824 versus 91 genes; −6.64 to 22.33-fold for hm-CGNversus the range of −2.65 to 2.96-fold for lm-CGN). Reporter systems with segments of the IL-8 promoter showed a specific activation in response to hm-sulfated polysaccharides with lower pathophysiological potential in vivo and provided a better classification of CGN-variants than cytotoxicity assays in vitro. IL-8 reporter systems can be used for discerning between the effects of sulfated polysaccharidesin vivo. Our data further provide initial insights into the molecular mechanisms that may play a role in the different effects of CGN-variants.
Starting Page	973
Ending Page	979
Page Count	7
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	5
Issue Number	9
Journal	Molecular BioSystems
DOI	10.1039/b901882b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Thickening agent Reporter gene DMBT1 Gene Cytotoxicity Carrageenan Polysaccharide Interleukin 8
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells.

Carrageenan inhibits granzyme A-induced detachment of and interleukin-8 release from alveolar epithelial A549 cells

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