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Alginate modified nanostructured calcium carbonate with enhanced delivery efficiency for gene and drug delivery

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Back cover

Molecular BioSystems Vol - 8 Issue - 2

Molecular BioSystems Vol -7

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Alginate modified nanostructured calcium carbonate with enhanced delivery efficiency for gene and drug delivery

Content Provider	Royal Society of Chemistry (RSC)
Author	Zhao, Dong Zhuo, Ren-Xi Cheng, Si-Xue
Copyright Year	2012
Abstract	To improve the performance of nanostructured calcium carbonate in gene delivery, a hydrophilic polysaccharide, alginate, was added to calcium carbonate co-precipitation systems to form alginate/CaCO3/DNA nanoparticles. The size and ζ-potential of the nanoparticles were measured by a zetasizer. Due to the existence of alginate chains which retarded the growth of calcium carbonate based co-precipitates, the alginate/CaCO3/DNA nanoparticles exhibited a decreased size and enhanced stability in the aqueous solution. To evaluate the gene and drug co-delivery ability, doxorubicin hydrochloride (DOX), a water-soluble anticancer drug, was loaded in the nanoparticles to form alginate/CaCO3/DNA/DOX nanoparticles. The in vitrogene transfections mediated by different nanoparticles in 293 T cells and HeLa cells were carried out, using pGL3-Luc as a reporter plasmid. With an appropriate amount of alginate, the gene transfection efficiency of alginate modified nanoparticles could be significantly enhanced as compared with the nanoparticles without alginate modification for the gene delivery systems, as well as the gene and drug co-delivery systems. The study on in vitrocell inhibition effects showed that the cell viability decreased with increasing DOX amount loaded in alginate/CaCO3/DNA/DOX nanoparticles. The alginate modification is a useful strategy to improve the calcium carbonate co-precipitation technique for the preparation of gene and drug delivery systems, and the nanoparticles prepared in this study have promising applications in gene and drug delivery.
Starting Page	753
Ending Page	759
Page Count	7
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	8
Issue Number	3
Journal	Molecular BioSystems
DOI	10.1039/c1mb05337j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Alginic acid Drug delivery Doxorubicin HeLa Plasmid Reporter gene Transfection Gene DNA Calcium carbonate Hydrophile Polysaccharide Coprecipitation
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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