NDLI: Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis

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Back matter

Back cover

Molecular BioSystems Vol - 7 Issue - 5

Molecular BioSystems Vol - 7 Issue - 4

Molecular BioSystems Vol - 7 Issue - 3

Molecular BioSystems Vol - 7 Issue - 2

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Systematic proteomic analysis of human hepotacellular carcinoma cells reveals molecular pathways and networks involved in metastasis

Content Provider	Royal Society of Chemistry (RSC)
Author	Yu, Hongxiu Zhang, Yang Liu, Yinkun Zhao, Jian Li, Hong Yu, Yanyan Shen, Huali Zhong, Fan Zhang, Chen Chen, Jie Yang, Pengyuan
Copyright Year	2011
Abstract	Systematic proteomic studying of the mechanism of hepatocellular carcinoma (HCC) metastasis remains challenging. We performed comparative proteomic and pathway analysis of four human metastatic HCC cell lines to identify metastasis-associated proteins. These HCC cell lines had a similar genetic background but with an increasing potential of metastasis. Using a combination of two dimensional electrophoresis (2-DE) and MALDI-TOF mass spectrometry, a total of 125 proteins and their post-translational modification forms or isoforms were found to be differentially expressed in the cell lines. Among them, 29 were gradually up-regulated whereas 17 were down-regulated with increasing metastatic potential. Instead of a traditional single-gene readout, global bioinformatics analysis was carried out, which revealed that the glycolysis pathway was the most significantly enriched pathway. The heat shock proteins (HSPs) centered and NF-kappaB centered networks were also enriched in the result, which may imply the key function of inflaming on metastasis. Meanwhile, knockdown of HDGF, an up-regulated protein and a target of NF-kappaB, induced cell apoptosis in the metastatic HCC cells. This work provides a demonstration that a combination of bioinformatics and comparative proteomics can help in finding out potential biomarkers associated with HCC metastasis on the level of pathways.
Starting Page	1908
Ending Page	1916
Page Count	9
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	7
Issue Number	6
Journal	Molecular BioSystems
DOI	10.1039/c0mb00265h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Post-translational modification Carcinoma Gel electrophoresis Hepatoma-derived growth factor HCC Metastasis Protein NF-κB Ionization Heat shock protein Proteomics Glycolysis Mass spectrometry Bioinformatics Apoptosis
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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