NDLI: Comparative analysis on the membrane proteome of Clostridium acetobutylicum wild type strain and its butanol-tolerant mutant

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Molecular BioSystems Vol - 7 Issue - 4

Molecular BioSystems Vol - 7 Issue - 3

Molecular BioSystems Vol - 7 Issue - 2

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Comparative analysis on the membrane proteome of Clostridium acetobutylicum wild type strain and its butanol-tolerant mutant

Content Provider	Royal Society of Chemistry (RSC)
Author	Ma, Yanhe Zhang, Yanping Mao, Shaoming Bao, Guanhui Luo, Yuanming Li, Yin
Copyright Year	2011
Abstract	The solventogenic bacterium Clostridium acetobutylicum is the most important species of Clostridium used in the fermentation industry. However, the intolerance to butanol hampers the efficient production of solvents. Butanol toxicity has been attributed to the chaotropic effect on the cell membrane, but the knowledge on the effect of butanol on membrane associated proteins is quite limited. Using 2-DE combined with MALDI-TOF MS/MS and 1-DE integrated with LC-MS/MS, 341 proteins in the membrane fractions of cell lysate were identified, thus establishing the first comprehensive membrane proteome of C. acetobutylicum. The identified proteins are mainly involved in transport, cellular membrane/wall machinery, formation of surface coat and flagella, and energy metabolism. Comparative analysis on the membrane proteomes of the wild type strain DSM 1731 and its butanol-tolerant mutant Rh8 revealed 73 differentially expressed proteins. Hierarchical clustering analysis suggested that mutant Rh8 may have evolved a more stabilized membrane structure, and have developed a cost-efficient energy metabolism strategy, to cope with the butanol challenge. This comparative membrane proteomics study, together with our previous published work on comparative cytoplasmic proteomics, allows us to obtain a systemic understanding of the effect of butanol on cellular physiology of C. acetobutylicum.
Starting Page	1660
Ending Page	1677
Page Count	18
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	7
Issue Number	5
Journal	Molecular BioSystems
DOI	10.1039/c0mb00330a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Clostridium Cell membrane Flagellum Cell biology Proteome Fermentation Metabolism Hierarchical clustering Ionization Proteomics Butanol Mutation DSM
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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