NDLI: Real time RNA transcription monitoring by Thiazole Orange (TO)-conjugated Peptide Nucleic Acid (PNA) probes: norovirus detection

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Back cover

Molecular BioSystems Vol - 7 Issue - 4

Molecular BioSystems Vol - 7 Issue - 3

Molecular BioSystems Vol - 7 Issue - 2

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Real time RNA transcription monitoring by Thiazole Orange (TO)-conjugated Peptide Nucleic Acid (PNA) probes: norovirus detection

Content Provider	Royal Society of Chemistry (RSC)
Author	Sforza, Stefano Tonelli, Alessandro Medici, Maria Cristina Chezzi, Carlo Corradini, Roberto Marchelli, Rosangela Tedeschi, Tullia Germini, Andrea
Copyright Year	2011
Abstract	Thiazole Orange (TO)-conjugated Peptide Nucleic Acid (PNA) probes have been reported as a valuable strategy for DNA analysis; however, no investigations targeting RNA molecules and no comparisons between different derivatization approaches have been reported so far. In this work, two TO-conjugated PNAs for genogroup II noroviruses (NoV GII) detection were designed and synthesized. Both the probes target the most conserved stretch of nucleotides identified in the open reading frame 1–2 (ORF1–ORF2) junction region and differ for the dye conjugation strategy: one PNA is end-labelled with the TO molecule tethered by a linker; the other probe bears the TO molecule directly linked to the PNA backbone, replacing a conventional nucleobase. The spectroscopic properties of the two PNA probes were studied and their applicability to NoVs detection, using an isothermal assay, was investigated. Both probes showed good specificity and high fluorescence enhancement upon hybridization, especially targeting RNA molecules. Moreover, the two probes were successfully employed for NoVs detection from stool specimens in an isothermal-based amplification assay targeting RNA ‘amplicons’. The probes showed to be specific even in the presence of high concentrations of non-target RNA.
Starting Page	1684
Ending Page	1692
Page Count	9
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	7
Issue Number	5
Journal	Molecular BioSystems
DOI	10.1039/c0mb00353k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Isothermal process PNA RNA Cell nucleus ORF eins Staining Fluorescence Nucleobase Thiazole Orange Open reading frame DNA Thiazole Proceedings of the National Academy of Sciences of the United States of America
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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