NDLI: Biodegradable cross-linked poly(amino alcohol esters) based on LMW PEI for gene delivery

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Biodegradable cross-linked poly(amino alcohol esters) based on LMW PEI for gene delivery

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Molecular BioSystems Vol - 7 Issue - 3

Molecular BioSystems Vol - 7 Issue - 2

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Biodegradable cross-linked poly(amino alcohol esters) based on LMW PEI for gene delivery

Content Provider	Royal Society of Chemistry (RSC)
Author	Yu, Xiao-Qi Zhu, Wen Wang, Yu Zhang, Ji Yang, Wei-Han Li, Shuo Dai, Zhen-Hua
Copyright Year	2011
Abstract	Polyethylenimine (PEI, especially with Mw of 25 000) has been known as an efficient gene carrier and a gold standard of gene transfection due to its high transfection efficiency (TE). However, high concomitant cytotoxicity limited the application of PEI. In this report, several cationic polymers derived from low molecular weight (LMW) PEI (Mw 600) linked with diglycidyl adipate (DA–PEI) or its analogs (diglycidyl succinate, DS–PEI and diglycidyl oxalate, DO–PEI; D–PEIs for all 3 polymers) were prepared and characterized. GPC gave Mws of DA–PEI, DS–PEI and DO–PEI as 6861, 16 015 and 35 281, respectively. Moreover, degradation of the ester-containing DS–PEI was also confirmed by GPC. In addition, hydroxyls in these polymers could improve their water solubility. These polymers exhibited good ability to condense plasmid DNA into nanoparticles with the size of 120–250 nm. ζ-potentials of the polyplexes were found to be around +10–20 mV under weight ratios (polymer/DNA) from 0.5 to 32. Agarose gel retardation showed that DNA could be released from the polyplexes after being pre-incubated for 30 h. In vitro experiments were carried out and it was found that DS–PEI showed about 5 times of TE compared to that of the PEI/DNA polyplex under a weight ratio of 1 in A549 cells. Meanwhile, the cytotoxicity of D–PEIs assayed by MTT is lower than that of 25 kDa PEI in HEK293 cells. These results suggested that this series of PEI derivatives would be promising non-viral biodegradable vectors for gene delivery.
Starting Page	1254
Ending Page	1262
Page Count	9
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	7
Issue Number	4
Journal	Molecular BioSystems
DOI	10.1039/c0mb00339e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Biodegradation Gold standard Cytotoxicity Polymer MTT Plasmid Polyethylenimine Transfection Gene DNA Succinic acid GPC Molar mass distribution
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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