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Fluorescent labeling of membrane proteins on the surface of living cells by a self-catalytic glutathione S-transferase omega 1 tag

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Back cover

Molecular BioSystems Vol - 7 Issue - 3

Molecular BioSystems Vol - 7 Issue - 2

Molecular BioSystems Vol -6

Molecular BioSystems Vol -5

Molecular BioSystems Vol -4

Molecular BioSystems Vol -3

Molecular BioSystems Vol -2

Fluorescent labeling of membrane proteins on the surface of living cells by a self-catalytic glutathione S-transferase omega 1 tag

Content Provider	Royal Society of Chemistry (RSC)
Author	Ha, Hyung-Ho Chang, Young-Tae Son, Jyunghyun Lee, Jae-Jung
Copyright Year	2011
Abstract	Imaging a specific protein of interest in live cell has versatile applications in biological research. Recently, diverse chemical tags have been developed to overcome the limits of autofluorescence protein (FP) tags. However, current chemical methods still need to be progressed to compete with FPs in the scope of specificity and convenience in staining procedure. We report a novel protein tagging method that provides a convenient and specific fluorescent labeling of membrane proteins. Ω tag is developed by employing a mammalian enzymeglutathione sulfur-transferase omega 1 (GSTO1) and its partner dye, 5-bromomethyl fluorescein (BMF). Ω-tagged membrane proteins were labeled by BMF efficiently for live cell imaging and in-gel analysis. Endocytosis of epidermal growth factor receptor (EGFR) was successfully visualized by using this Ω tagging system. Ω tag will provide a convenient tool for the physiological study of membrane proteins in live cells.
Starting Page	1270
Ending Page	1276
Page Count	7
File Format	HTM / HTML PDF
ISSN	1742206X
Volume Number	7
Issue Number	4
Journal	Molecular BioSystems
DOI	10.1039/c0mb00327a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Endocytosis GSTO1 Cell surface receptor Autofluorescence Fluorescein Staining EGFR Protein Epidermal growth factor receptor Growth factor
Content Type	Text
Resource Type	Article
Subject	Molecular Biology Biotechnology

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Identification of glutathione S-transferase omega 1 (GSTO1) protein as a novel tumor-associated antigen and its autoantibody in human esophageal squamous cell carcinoma

Epidermal growth factor receptor distribution during (2000).

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Genetically engineered epidermal growth factor conjugate crosses cell membrane.

Endocytosis of Epidermal Growth Factor Receptor Regulated by Grb2-mediated Recruitment of the Rab5 GTPase-activating Protein RN-tre

Huntingtin-associated Protein 1 Interacts with Hepatocyte Growth Factor-regulated Tyrosine Kinase Substrate and Functions in Endosomal Trafficking

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Proteomics reveals novel protein associations with early endosomes in an epidermal growth factor–dependent manner

Fluorescent labeling of membrane proteins on the surface of living cells by a self-catalytic glutathione S-transferase omega 1 tag

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