NDLI: Large-scale speciation of selenium in rice proteins using ICP-MS assisted electrospray MS/MS proteomics

Content Provider	Royal Society of Chemistry (RSC)
Author	Lobinski, Ryszard Bianga, Juliusz Szpunar, Joanna Arnaudguilhem, Carine Cheajesadagul, Pracha
Copyright Year	2014
Abstract	A Se-targeted bottom-up proteomics approach was developed for the identification of Se-containing proteins in rice grown naturally on seleniferous soils. The proteins were separated by 2D gel electrophoresis. The position of Se-containing spots was tentatively identified by the correlation between the 1D isoelectrofocusing (IEF) and 1D SDS electropherograms of a sample aliquot and confirmed by 78Se imaging in the 2D gel. The method was complemented by the ICP-MS assisted shotgun proteomics approach. The proteins were identified by capHPLC with the dual ICP MS and electrospray Orbitrap MS detection. The first ever comprehensive study of rice selenoproteome revealed the presence of selenium, as both selenomethionine (SeMet) and selenocysteine (SeCys) residues, in a dozen proteins including a 19 kDa globulin, granule-bound starch synthase, and the family of glutelin-type seed storage proteins.
Starting Page	646
Ending Page	653
Page Count	8
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	6
Issue Number	3
Journal	Metallomics
DOI	10.1039/c3mt00299c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oryza sativa Electrospray ionization Two-dimensional gel electrophoresis Proteomics Selenocysteine Starch synthase Orbitrap Selenium Shotgun sequencing Bottom-up proteomics Globulin Selenomethionine
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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