NDLI: Analysis of animal and plant selenometabolites in roots of a selenium accumulator, Brassica rapa var. peruviridis, by speciation

Content Provider	Royal Society of Chemistry (RSC)
Author	Ogihara, Yurie Katayama, Ayane Anan, Yasumi Yawata, Ayako Ogra, Yasumitsu
Copyright Year	2013
Abstract	Many studies have examined the metabolic pathway of selenium (Se) compounds in Se-accumulating plants (hereafter “Se accumulators”) when the plants are exposed to inorganic Se, such as selenite and selenate. However, if we were to consider Se circulation in the biosphere, the metabolism of organic Se, in particular, selenometabolites of animals and plants, in plants should be elucidated. In this study, Brassica rapa var. peruviridis, a known Se accumulator, was hydroponically cultivated and then exposed to selenometabolites of animals and plants, such as methyl-2-acetamido-2-deoxy-1-seleno-β-D-galactopyranoside (selenosugar, SeSug), trimethylselenonium (TMSe), selenomethionine (SeMet), and Se-methylselenocysteine (MeSeCys). Then, the metabolic pathway of the organic Se compounds/selenometabolites in B. rapa var. peruviridis was investigated by speciation analysis. Two selenometabolites were detected in the roots when the plant was exposed to SeMet, MeSeCys, and SeSug. They were assigned to S-(methylseleno)-glutathione and MeSeCys using electrospray tandem mass spectrometry (ESI-MS-MS) and HPLC-inductively coupled plasma mass spectrometry (ICP-MS). Contrary to SeMet, MeSeCys, and SeSug, TMSe was not metabolized even if it was more efficiently incorporated into the roots than the other Se compounds. The identified metabolites enabled us to propose a metabolic pathway for the organic Se metabolites except TMSe in the plant roots: a monomethylseleno moiety (CH3Se–) commonly existing in SeMet, MeSeCys, and SeSug was cleaved off and conjugated with GSH, and then the CH3Se group was transferred to O-acetylserine to form MeSeCys.
Starting Page	429
Ending Page	436
Page Count	8
File Format	HTM / HTML PDF
ISSN	17565901
Volume Number	5
Issue Number	5
Journal	Metallomics
DOI	10.1039/c2mt20187a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Brassica rapa Electrospray ionization Methylselenocysteine Metabolic pathway Selenate Metabolism Blood plasma Brassica Selenium Rapa Iti Mass spectrometry Speciation Selenomethionine Glutathione
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Metals and Alloys Biochemistry Biomaterials Biophysics

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