NDLI: Integrated multilayer microfluidic device with a nanoporous membrane interconnect for online coupling of solid-phase extraction to microchip electrophoresis

Content Provider	Royal Society of Chemistry (RSC)
Author	Qin, Jianhua Shen, Zheng Wu, Dapeng Long, Zhicheng Lin, Bingcheng
Copyright Year	2007
Abstract	An integrated microfluidic device was developed for online coupling of solid-phase extraction to microchip electrophoresis (chip SPE-CE). With a nanoporous membrane sandwiched between two PDMS substrates, SPE preconcentration and electrophoretic separation can be carried out in upper and lower fluidic layers, separately and sequentially. During the SPE process, the thin membrane can act as a fluid isolator to prevent intermixing between two fluidic channels. However, when a pulse voltage is applied, the membrane becomes a gateable interconnect so that a small plug of concentrated analytes can be online injected into the lower channel for subsequent separations. This multilayer design provides a universal solution to online SPE-CE hyphenation. Both electroosmotic flow and hydrodynamic pumps have been adopted for SPE operation. SPE was performed on a 2.5 mm long microcolumn, with two weirs on both sides to retain the C18-coated silica beads. Rhodamine 123 and FITC-labelled ephedrine were used to test the operational performance of the hyphenation system. High separation efficiency and thousand-fold signal enhancement were achieved.
Starting Page	1819
Ending Page	1824
Page Count	6
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	12
Journal	Lab on a Chip
DOI	10.1039/b711741h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Solid phase extraction Rhodamine Electro-osmosis Field-effect transistor Integrated circuit Ephedrine Electrophoresis Silicon dioxide
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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