NDLI: Matrix-dependent adhesion of vascular and valvular endothelial cells in microfluidic channels

Content Provider	Royal Society of Chemistry (RSC)
Author	Young, Edmond W. K. Wheeler, Aaron R. Simmons, Craig A.
Copyright Year	2007
Abstract	The interactions between endothelial cells and the underlying extracellular matrix regulate adhesion and cellular responses to microenvironmental stimuli, including flow-induced shear stress. In this study, we investigated the adhesion properties of primary porcine aortic endothelial cells (PAECs) and valve endothelial cells (PAVECs) in a microfluidic network. Taking advantage of the parallel arrangement of the microchannels, we compared adhesion of PAECs and PAVECs to fibronectin and type I collagen, two prominent extracellular matrix proteins, over a broad range of concentrations. Cell spreading was measured morphologically, based on cytoplasmic staining with a vital dye, while adhesion strength was characterized by the number of cells attached after application of shear stresses of 11, 110, and 220 dyn cm−2. Results showed that PAVECs were more well spread on fibronectin than on type I collagen (P < 0.0001), particularly for coating concentrations of 100, 200, and 500 µg mL−1. PAVECs also withstood shear significantly better on fibronectin than on collagen for 500 µg mL−1. PAECs were more well spread on collagen compared to PAVECs (P < 0.0001), but did not have significantly better adhesion strength. These results demonstrate that cell adhesion is both cell-type and matrix dependent. Furthermore, they reveal important phenotypic differences between vascular and valvular endothelium, with implications for endothelial mechanobiology and the design of microdevices and engineered tissues.
Starting Page	1759
Ending Page	1766
Page Count	8
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	7
Issue Number	12
Journal	Lab on a Chip
DOI	10.1039/b712486d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Microchannel (microtechnology) Collagen Cell adhesion Mechanobiology Shear stress Staining Extracellular matrix Aorta Type I collagen Fibronectin Endothelium
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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