NDLI: Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis

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Automated microfluidic devices integrating solid-phase extraction, fluorescent labeling, and microchip electrophoresis for preterm birth biomarker analysis

Content Provider	Semantic Scholar
Author	Sahore, Vishal Sonker, Mukul Nielsen, Anna V. Knob, Radim Kumar, Suresh Woolley, Adam T.
Copyright Year	2017
Abstract	AbstractWe have developed multichannel integrated microfluidic devices for automated preconcentration, labeling, purification, and separation of preterm birth (PTB) biomarkers. We fabricated multilayer poly(dimethylsiloxane)-cyclic olefin copolymer (PDMS-COC) devices that perform solid-phase extraction (SPE) and microchip electrophoresis (μCE) for automated PTB biomarker analysis. The PDMS control layer had a peristaltic pump and pneumatic valves for flow control, while the PDMS fluidic layer had five input reservoirs connected to microchannels and a μCE system. The COC layers had a reversed-phase octyl methacrylate porous polymer monolith for SPE and fluorescent labeling of PTB biomarkers. We determined μCE conditions for two PTB biomarkers, ferritin (Fer) and corticotropin-releasing factor (CRF). We used these integrated microfluidic devices to preconcentrate and purify off-chip-labeled Fer and CRF in an automated fashion. Finally, we performed a fully automated on-chip analysis of unlabeled PTB biomarkers, involving SPE, labeling, and μCE separation with 1 h total analysis time. These integrated systems have strong potential to be combined with upstream immunoaffinity extraction, offering a compact sample-to-answer biomarker analysis platform. Graphical abstractPressure-actuated integrated microfluidic devices have been developed for automated solid-phase extraction, fluorescent labeling, and microchip electrophoresis of preterm birth biomarkers
Starting Page	933
Ending Page	941
Page Count	9
File Format	PDF HTM / HTML
DOI	10.1007/s00216-017-0548-7
PubMed reference number	28799040
Journal	Medline
Volume Number	410
Alternate Webpage(s)	https://static-content.springer.com/esm/art:10.1007%2Fs00216-017-0548-7/MediaObjects/216_2017_548_MOESM1_ESM.pdf
Alternate Webpage(s)	https://doi.org/10.1007/s00216-017-0548-7
Journal	Analytical and Bioanalytical Chemistry
Language	English
Access Restriction	Open
Content Type	Text
Resource Type	Article

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