NDLI: A multifunctional micro-fluidic system for dielectrophoretic concentration coupled with immuno-capture of low numbers of Listeria monocytogenes

Content Provider	Royal Society of Chemistry (RSC)
Author	Chatni, Mohammad R. Bhunia, Arun K. Banada, Padmapriya P. Bashir, Rashid Yang, Liju Ladisch, Michael Lim, Kwan Seop
Copyright Year	2006
Abstract	In this study, we demonstrated a micro-fluidic system with multiple functions, including concentration of bacteria using dielectrophoresis (DEP) and selective capture using antibody recognition, resulting in a high capture efficiency of bacterial cells. The device consisted of an array of oxide covered interdigitated electrodes on a flat silicon substrate and a ∼16 µm high and ∼260 µm wide micro-channel within a PDMS cover. For selective capture of Listeria monocytogenes from the samples, the channel surface was functionalized with a biotinylated BSA–streptavidin–biotinylated monoclonal antibody sandwich structure. Positive DEP (at 20 Vpp and 1 MHz) was used to concentrate bacterial cells from the fluid flow. DEP could collect ∼90% of the cells in a continuous flow at a flow rate of 0.2 µl min−1 into the micro-channel with concentration factors between 102–103, in sample volumes of 5–20 µl. A high flow rate of 0.6 µl min−1 reduced the DEP capture efficiency to ∼65%. Positive DEP attracts cells to the edges of the electrodes where the field gradient is the highest. Cells concentrated by DEP were captured by the antibodies immobilized on the channel surface with efficiencies of 18 to 27% with bacterial cell numbers ranging from 101 to 103 cells. It was found that DEP operation in our experiments did not cause any irreversible damage to bacterial cells in terms of cell viability. In addition, increased antigen expression (antigens to C11E9 monoclonal antibody) on cell membranes was observed following the exposure to DEP.
Starting Page	896
Ending Page	905
Page Count	10
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	7
Journal	Lab on a Chip
DOI	10.1039/b607061m
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Antigen BSA Sandwich compound Antibody Micrometre Listeria monocytogenes Bacteria Monoclonal antibody DEP Channel surface
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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