NDLI: DC-dielectrophoretic separation of microparticles using an oil droplet obstacle

Content Provider	Royal Society of Chemistry (RSC)
Author	Barbulovic-Nad, Irena Lee, Jacky S. H. Li, Dongqing Xuan, Xiangchun
Copyright Year	2006
Abstract	A new dielectrophoretic particle separation method is demonstrated and examined in the following experimental study. Current electrodeless dielectrophoretic (DEP) separation techniques utilize insulating solid obstacles in a DC or low-frequency AC field, while this novel method employs an oil droplet acting as an insulating hurdle between two electrodes. When particles move in a non-uniform DC field locally formed by the droplet, they are exposed to a negative DEP force linearly dependent on their volume, which allows the particle separation by size. Since the size of the droplet can be dynamically changed, the electric field gradient, and hence DEP force, becomes easily controllable and adjustable to various separation parameters. By adjusting the droplet size, particles of three different diameter sizes, 1 µm, 5.7 µm and 15.7 µm, were successfully separated in a PDMS microfluidic chip, under applied field strength in the range from 80 V cm−1 to 240 V cm−1. A very effective separation was realized at the low field strength, since the electric field gradient was proved to be a more significant parameter for particle discrimination than the applied voltage. By utilizing low strength fields and adaptable field gradient, this method can also be applied to the separation of biological samples that are generally very sensitive to high electric potential.
Starting Page	274
Ending Page	279
Page Count	6
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	2
Journal	Lab on a Chip
DOI	10.1039/b513183a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Electric potential Electric field Micrometre DEP DC
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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