NDLI: Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification

Content Provider	Royal Society of Chemistry (RSC)
Author	Lee, Jeong-Gun Huh, Nam Kim, Suhyeon Cheong, Kwang Ho Choi, Jeong-Woo Ko, Christopher
Copyright Year	2006
Abstract	Optimal detection of a pathogen present in biological samples depends on the ability to extract DNA molecules rapidly and efficiently. In this paper, we report a novel method for efficient DNA extraction and subsequent real-time detection in a single microchip by combining laser irradiation and magnetic beads. By using a 808 nm laser and carboxyl-terminated magnetic beads, we demonstrate that a single pulse of 40 seconds lysed pathogens including E. coli and Gram-positive bacterial cells as well as the hepatitis B virus mixed with human serum. We further demonstrate that the real-time pathogen detection was performed with pre-mixed PCR reagents in a real-time PCR machine using the same microchip, after laser irradiation in a hand-held device equipped with a small laser diode. These results suggest that the new sample preparation method is well suited to be integrated into lab-on-a-chip application of the pathogen detection system.
Starting Page	886
Ending Page	895
Page Count	10
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	7
Journal	Lab on a Chip
DOI	10.1039/b515876a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	Polymerase chain reaction DNA extraction Diode DNA Escherichia coli Integrated circuit Laser Gram-positive bacteria Lab-on-a-chip Hepatitis B virus Pathogen
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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