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Microchip-based one step DNA extraction and real-time PCR in one chamber for rapid pathogen identification.
| Content Provider | Semantic Scholar |
|---|---|
| Author | Lee, Jeong-Gun Cheong, Kwang Ho Kim, Suhyeon Choi, Jeong-Woo Ko, Christopher |
| Copyright Year | 2006 |
| Abstract | Optimal detection of a pathogen present in biological samples depends on the ability to extract DNA molecules rapidly and efficiently. In this paper, we report a novel method for efficient DNA extraction and subsequent real-time detection in a single microchip by combining laser irradiation and magnetic beads. By using a 808 nm laser and carboxyl-terminated magnetic beads, we demonstrate that a single pulse of 40 seconds lysed pathogens including E. coli and Gram-positive bacterial cells as well as the hepatitis B virus mixed with human serum. We further demonstrate that the real-time pathogen detection was performed with pre-mixed PCR reagents in a real-time PCR machine using the same microchip, after laser irradiation in a hand-held device equipped with a small laser diode. These results suggest that the new sample preparation method is well suited to be integrated into lab-on-a-chip application of the pathogen detection system. |
| File Format | PDF HTM / HTML |
| DOI | 10.1039/B515876A |
| Alternate Webpage(s) | http://qpcrupdate.com/lee-lab-on-chip-2006.pdf |
| Alternate Webpage(s) | http://gene-quantification.com/lee-lab-on-chip-2006.pdf |
| PubMed reference number | 16804593 |
| Alternate Webpage(s) | https://doi.org/10.1039/B515876A |
| Journal | Medline |
| Volume Number | 6 |
| Issue Number | 7 |
| Journal | Lab on a chip |
| Language | English |
| Access Restriction | Open |
| Content Type | Text |
| Resource Type | Article |
