NDLI: Characterization of the local temperature in space and time around a developing Drosophila embryo in a microfluidic device

Content Provider	Royal Society of Chemistry (RSC)
Author	Munson, Matthew S. Lucchetta, Elena M. Ismagilov, Rustem F.
Copyright Year	2006
Abstract	This paper characterizes a microfluidic platform that differentially controls the temperature of each half of a living Drosophila melanogaster fruitfly embryo in space and time (E. M. Lucchetta, J. H. Lee, L. A. Fu, N. H. Patel and R. F. Ismagilov, Nature, 2005, 434, 1134–1138). This platform relies on laminar flow of two streams of liquid with different temperature, and on rapid prototyping in polydimethylsiloxane (PDMS). Here, we characterized fluid flow and heat transport in this platform both experimentally and by numerical simulation, and estimated the temperature distribution around and within the embryo by numerical simulation, to identify the conditions for creating a sharper temperature difference (temperature step) over the embryo. Embryos were removed from the device and immunostained histochemically for detection of Paired protein. Biochemical processes are sensitive to small differences in environmental temperature. The microfluidic platform characterized here could prove useful in understanding dynamics of biochemical networks as they respond to changes in temperature.
Starting Page	185
Ending Page	190
Page Count	6
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	6
Issue Number	2
Journal	Lab on a Chip
DOI	10.1039/b516119c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Nature Embryo Lucchetta Ismagilov Lee Protein Fu Laminar flow Robert E. Lee Rapid prototyping Drosophila melanogaster Polydimethylsiloxane Patel
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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