NDLI: Resealable, optically accessible, PDMS-free fluidic platform for ex vivo interrogation of pancreatic islets

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Inside back cover

Back cover

Lab on a Chip Vol - 17 Issue - 4

Lab on a Chip Vol - 17 Issue - 3

Lab on a Chip Vol - 17 Issue - 2

Lab on a Chip Vol - 17 Issue - 1

Resealable, optically accessible, PDMS-free fluidic platform for ex vivo interrogation of pancreatic islets

Content Provider	Royal Society of Chemistry (RSC)
Author	Chaimov, Deborah Stabler, Cherie L. Caicedo, Alejandro Rodriguez-Diaz, Rayner Lenguito, Giovanni Weitz, Jonathan R. Tamayo-Garcia, Alejandro Rawal, Siddarth A. K. Buchwald, Peter Agarwal, Ashutosh
Copyright Year	2017
Abstract	We report the design and fabrication of a robust fluidic platform built out of inert plastic materials and micromachined features that promote optimized convective fluid transport. The platform is tested for perfusion interrogation of rodent and human pancreatic islets, dynamic secretion of hormones, concomitant live-cell imaging, and optogenetic stimulation of genetically engineered islets. A coupled quantitative fluid dynamics computational model of glucose stimulated insulin secretion and fluid dynamics was first utilized to design device geometries that are optimal for complete perfusion of three-dimensional islets, effective collection of secreted insulin, and minimization of system volumes and associated delays. Fluidic devices were then fabricated through rapid prototyping techniques, such as micromilling and laser engraving, as two interlocking parts from materials that are non-absorbent and inert. Finally, the assembly was tested for performance using both rodent and human islets with multiple assays conducted in parallel, such as dynamic perfusion, staining and optogenetics on standard microscopes, as well as for integration with commercial perfusion machines. The optimized design of convective fluid flows, use of bio-inert and non-absorbent materials, reversible assembly, manual access for loading and unloading of islets, and straightforward integration with commercial imaging and fluid handling systems proved to be critical for perfusion assay, and particularly suited for time-resolved optogenetics studies.
Starting Page	772
Ending Page	781
Page Count	10
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	17
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/c6lc01504b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Fluid dynamics Perfusion Rapid prototyping Pancreatic islets Laser engraving Optogenetics Glucose Rodent Insulin
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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