NDLI: High-selectivity cytology via lab-on-a-disc western blotting of individual cells

Please wait, while we are loading the content...

Reproducible in vitro model for dystrophic calcification of cardiac valvular interstitial cells: insights into the mechanisms of calcific aortic valvular disease

A nano flow cytometer for single lipid vesicle analysis

Modulating motility of intracellular vesicles in cortical neurons with nanomagnetic forces on-chip

High-selectivity cytology via lab-on-a-disc western blotting of individual cells

Robust temperature change rate actuated valving and switching for highly integrated centrifugal microfluidics

Cell agglomeration in the wells of a 24-well plate using acoustic streaming

Microfluidic platform for rapid measurement of transepithelial water transport

A 3D microblade structure for precise and parallel droplet splitting on digital microfluidic chips

Improved bovine embryo production in an oviduct-on-a-chip system: prevention of poly-spermic fertilization and parthenogenic activation

Disposable cartridge platform for rapid detection of viral hemorrhagic fever viruses

Wearable microfluidics: fabric-based digital droplet flowmetry for perspiration analysis

Tandem emulsification for high-throughput production of double emulsions

Automating multi-step paper-based assays using integrated layering of reagents

Determination of the temperature-dependent cell membrane permeabilities using microfluidics with integrated flow and temperature control

Correction: The magnitude of lift forces acting on drops and bubbles in liquids flowing inside microchannels

Inside back cover

Back cover

Lab on a Chip Vol - 17 Issue - 4

Lab on a Chip Vol - 17 Issue - 3

Lab on a Chip Vol - 17 Issue - 2

Lab on a Chip Vol - 17 Issue - 1

High-selectivity cytology via lab-on-a-disc western blotting of individual cells

Content Provider	Royal Society of Chemistry (RSC)
Author	Herr, Amy E. Sinkala, Elly Kim, John J.
Copyright Year	2017
Abstract	Cytology of sparingly available cell samples from both clinical and experimental settings would benefit from high-selectivity protein tools. To minimize cell handling losses in sparse samples, we design a multi-stage assay using a lab-on-a-disc that integrates cell handling and subsequent single-cell western blotting (scWestern). As the two-layer microfluidic device rotates, the induced centrifugal force directs dissociated cells to dams, which in turn localize the cells over microwells. Cells then sediment into the microwells, where the cells are lysed and subjected to scWestern. Taking into account cell losses from loading, centrifugation, and lysis-buffer exchange, our lab-on-a-disc device handles cell samples with as few as 200 cells with 75% cell settling efficiencies. Over 70% of microwells contain single cells after the centrifugation. In addition to cell settling efficiency, cell-size filtration from a mixed population of two cell lines is also realized by tuning the cell time-of-flight during centrifugation (58.4% settling efficiency with 6.4% impurity). Following the upstream cell handling, scWestern analysis detects four proteins (GFP, β-TUB, GAPDH, and STAT3) in a glioblastoma cell line. By integrating the lab-on-a-disc cell preparation and scWestern analysis, our platform measures proteins from sparse cell samples at single-cell resolution.
Starting Page	855
Ending Page	863
Page Count	9
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	17
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/c6lc01333c
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	GFP Western blot Glyceraldehyde 3-phosphate dehydrogenase Centrifugation Green fluorescent protein STAT3 Centrifugal force GAPDH Sedimentation Time-of-flight mass spectrometry Glioblastoma multiforme Protein
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Protein moonlighting in iron metabolism: glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death

Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase encoding gene inGracilaria/Gracilariopsis lemaneiformis

GC Box-binding Transcription Factors Control the Neuronal Specific Transcription of the Cyclin-dependent Kinase 5 Regulator p35

Splicing Factor SRp30c Interaction with Y-box Protein-1 Confers Nuclear YB-1 Shuttling and Alternative Splice Site Selection

Sperm-Specific Glyceraldehyde-3-Phosphate Dehydrogenase–An Evolutionary Acquisition of Mammals

Diurnal Pineal 3-O-Sulphotransferase 2 Expression Controlled by β-Adrenergic Repression

Immobilized D-glyceraldehyde-3-phosphate dehydrogenase can exist as a trimer.

High-selectivity cytology via lab-on-a-disc western blotting of individual cells

Similar Documents

Protein moonlighting in iron metabolism: glyceraldehyde-3-phosphate dehydrogenase (GAPDH).

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death

Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) Protein-Protein Interaction Inhibitor Reveals a Non-catalytic Role for GAPDH Oligomerization in Cell Death

Cloning and characterization of glyceraldehyde-3-phosphate dehydrogenase encoding gene inGracilaria/Gracilariopsis lemaneiformis

GC Box-binding Transcription Factors Control the Neuronal Specific Transcription of the Cyclin-dependent Kinase 5 Regulator p35

Splicing Factor SRp30c Interaction with Y-box Protein-1 Confers Nuclear YB-1 Shuttling and Alternative Splice Site Selection

Sperm-Specific Glyceraldehyde-3-Phosphate Dehydrogenase–An Evolutionary Acquisition of Mammals

Diurnal Pineal 3-O-Sulphotransferase 2 Expression Controlled by β-Adrenergic Repression

Immobilized D-glyceraldehyde-3-phosphate dehydrogenase can exist as a trimer.

High-selectivity cytology via lab-on-a-disc western blotting of individual cells