NDLI: Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip

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Inside back cover

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Lab on a Chip Vol - 17 Issue - 4

Lab on a Chip Vol - 17 Issue - 3

Lab on a Chip Vol - 17 Issue - 2

Lab on a Chip Vol - 17 Issue - 1

Mimicking liver sinusoidal structures and functions using a 3D-configured microfluidic chip

Content Provider	Royal Society of Chemistry (RSC)
Author	Long, Mian Li, Ning Gao, Yuxin Tong, Chunfang Gong, Yixin Yang, Hao Luo, Chunhua Lü, Shouqin Du, Yu
Copyright Year	2017
Abstract	Physiologically, four major types of hepatic cells – the liver sinusoidal endothelial cells, Kupffer cells, hepatic stellate cells, and hepatocytes – reside inside liver sinusoids and interact with flowing peripheral cells under blood flow. It is hard to mimic an in vivo liver sinusoid due to its complex multiple cell–cell interactions, spatiotemporal construction, and mechanical microenvironment. Here we developed an in vitro liver sinusoid chip by integrating the four types of primary murine hepatic cells into two adjacent fluid channels separated by a porous permeable membrane, replicating liver's key structures and configurations. Each type of cells was identified with its respective markers, and the assembled chip presented the liver-specific unique morphology of fenestration. The flow field in the liver chip was quantitatively analyzed by computational fluid dynamics simulations and particle tracking visualization tests. Intriguingly, co-culture and shear flow enhance albumin secretion independently or cooperatively, while shear flow alone enhances HGF production and CYP450 metabolism. Under lipopolysaccharide (LPS) stimulations, the hepatic cell co-culture facilitated neutrophil recruitment in the liver chip. Thus, this 3D-configured in vitro liver chip integrates the two key factors of shear flow and the four types of primary hepatic cells to replicate key structures, hepatic functions, and primary immune responses and provides a new in vitro model to investigate the short-duration hepatic cellular interactions under a microenvironment mimicking the physiology of a liver.
Starting Page	782
Ending Page	794
Page Count	13
File Format	HTM / HTML PDF
ISSN	14730197
Volume Number	17
Issue Number	5
Journal	Lab on a Chip
DOI	10.1039/c6lc01374k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Computational fluid dynamics Hepatocyte growth factor Liver Albumin Cell biology Liver sinusoid Metabolism Kupffer cell Hepatocyte Sinusoid (blood vessel) Lipopolysaccharide Drug interaction Neutrophil
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Biochemistry Bioengineering Biomedical Engineering

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