NDLI: A novel gene carrier prepared from triple helical β-glucan and polydeoxyadenylic acid

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Journal of Materials Chemistry B Vol - 2 Issue - 7

Journal of Materials Chemistry B Vol - 2 Issue - 6

Journal of Materials Chemistry B Vol - 2 Issue - 5

Journal of Materials Chemistry B Vol -1

A novel gene carrier prepared from triple helical β-glucan and polydeoxyadenylic acid

Content Provider	Royal Society of Chemistry (RSC)
Author	Cao, Yan Liu, Qingye Wang, Chaoqun Xu, Xiaojuan Zhang, Lina
Copyright Year	2014
Abstract	A naturally occurring β-glucan from Lentinus edodes (t-LNT) has been proved to adopt a unique triple helix structure due to strong hydrogen bonding. The single chain of t-LNT (s-LNT) can interact with polydeoxyadenylic acid (poly(dA)) through hydrogen bonding between s-LNT and poly(dA). Based on this, a novel strategy for an efficient gene transfection was developed as follows. The target DNA was attached to poly(dA)50 through a disulfide bond to form DNA–SS–poly(dA)50, and s-LNT was then combined with the poly(dA)50 segment to form a DNA–SS–poly(dA)50/s-LNT complex through hydrogen bonding, which could be taken up by cells followed by the release of target DNA in the cytoplasm via the breaking of the disulfide bond under the treatment of reducing agents such as GSH. The experimental results from the agarose gel retardation assay and circular dichroism demonstrated the formation of the complex DNA–SS–poly(dA)50/s-LNT. Confocal microscopy and flow cytometry indicated that the target DNA was delivered into the cells with high transfection efficiency, which was strongly dependent on molecular weight and the concentration of s-LNT. The uptake mechanism of the complex was ascribed to endocytosis. The significant enhancement of IL-12p40 production in macrophage RAW 264.7 cells stimulated by the transfected CpG DNA with immunomodulating activity further confirmed the feasibility of this system. Additionally, gene transfection with high efficiency could be performed in tumor cells (HeLa). The cytotoxicity of s-LNT against RAW 264.7 and HeLa cells by the MTT assay showed good safety. Overall, s-LNT could be used as an excellent gene carrier, indicating its promising application in gene delivery.
Starting Page	933
Ending Page	944
Page Count	12
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	2
Issue Number	8
Journal	Journal of Materials Chemistry B
DOI	10.1039/c3tb21195a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	MTT Shiitake Hydrogen bond Interaction Carboxylic acid Gene Transfection DNA Disulfide Cytoplasm Agarose Circular dichroism Confocal microscopy Flow cytometry The UpTake Endocytosis Interleukin-12 subunit beta Macrophage Raw image format CpG site HeLa Cytotoxicity MTT assay
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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