NDLI: Double entrapment of growth factors by nanoparticles loaded into polyelectrolyte multilayer films

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Journal of Materials Chemistry B Vol - 2 Issue - 7

Journal of Materials Chemistry B Vol - 2 Issue - 6

Journal of Materials Chemistry B Vol - 2 Issue - 5

Journal of Materials Chemistry B Vol -1

Double entrapment of growth factors by nanoparticles loaded into polyelectrolyte multilayer films

Content Provider	Royal Society of Chemistry (RSC)
Author	Keskin, D. Francius, G. Lavalle, P. Erdemli, O. Fahs, A. Rabineau, M. Tezcaner, A. Debry, C. Vrana, N. E.
Copyright Year	2014
Abstract	Delivery of growth factors and control of vascularization are prominent problems in regenerative medicine. Vascular endothelial growth factor (VEGF) has been used both in vitro and in vivo to promote angiogenesis but due to its short half-life its controlled delivery is a sought after method. In this study we present a new concept of degradable drug loaded nanoparticles entrapped into exponentially growing multilayer films. Through hydrolysis of the nanoparticles, the drug can be delivered over long periods in a controlled manner. Poly(ε-caprolactone) nanoparticles were loaded with VEGF and in turn the release of VEGF from a surface is controlled by a thick layer-by-layer polyelectrolyte film. Direct loading of VEGF inside the film was not efficient for long-term applications. When VEGF loaded nanoparticles were introduced into the film, the particles were equally distributed inside and were stable after several washes. Moreover, the presence of the film sustained the release of VEGF for 7 days. Addition of the nanoparticles to the film promoted endothelial cell proliferation, mainly due to the presence of VEGF. Mechanical properties of the film (Young's moduli) were also improved by the presence of nanoparticles. However, in the presence of the film loaded with nanoparticles and without any direct contact with this film, endothelial cell growth was also enhanced on polystyrene and on Transwell insert surfaces which demonstrates the effectiveness of the nanoparticles not only to improve the mechanical properties of the film but also to deliver active VEGF. An increase in nitric oxide levels as an indicator of endothelial cell activity was monitored and was correlated with the release of VEGF from the nanoparticle/film platform. Finally, such a system can be used as an auxiliary delivery body within implants to finely control the release of bioactive agent containing nanoparticles.
Starting Page	999
Ending Page	1008
Page Count	10
File Format	HTM / HTML PDF
ISSN	2050750X
Volume Number	2
Issue Number	8
Journal	Journal of Materials Chemistry B
DOI	10.1039/c3tb21304h
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Wound healing Vascular endothelial growth factor Growth factor Angiogenesis Half-life Hydrolysis Caprolactone Polyelectrolyte Endothelium Direct Contact Polystyrene Nitric oxide Nanoparticle
Content Type	Text
Resource Type	Article
Subject	Chemistry Medicine Materials Science Biomedical Engineering

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