NDLI: Peptides present in the non-digestible fraction of common beans (<i>Phaseolus vulgaris</i> L.) inhibit the angiotensin-I converting enzyme by interacting with its catalytic cavity independent of their antioxidant capacity

Content Provider	Royal Society of Chemistry (RSC)
Author	Luna-Vital, Diego A. Loarca-Piña, Guadalupe Mejía, Elvira González de Mendoza, Sandra
Copyright Year	2015
Abstract	The aim was to evaluate the angiotensin-I converting enzyme (ACE) inhibitory potential and the antioxidant capacity of pure synthesized peptides (GLTSK, LSGNK, GEGSGA, MPACGSS and MTEEY) originally identified in the non-digestible fraction (NDF) of common beans (P. vulgaris L.) that had previously demonstrated antiproliferative activity against human colorectal cancer cells. The five peptides were able to inhibit ACE with half maximal inhibitory concentration (IC50) values ranging from 65.4 (GLTSK) to 191.5 μM (MPACGSS). The combination of GLTSK and MTEEY increased the ACE inhibition by 30% compared to equieffective doses of the single peptides. According to molecular docking analysis, the five peptides had lower estimated free energy values (−6.47 to −9.34 kcal mol−1) when they interacted with the catalytic site of ACE than that of the substrate hippuryl-histidyl-leucine (−5.41 kcal mol−1), thus inhibiting the enzymatic activity. According to molecular docking analysis, the five peptides interacted with four (His353, Ala354, Glu411 and Tyr523) out of 6 catalytic residues. Moreover, MPACGSS had the highest antioxidant activity according to Ferric reducing antioxidant power (FRAP) (421.58 μmol FeSO4 mg−1), Fe2+ chelation (2.01 μmol Na2EDTA mg−1) assays, and also in DPPH (748.39 μmol Trolox per mg of dry peptide) and ABTS (561.42 μmol Trolox mg−1) radical scavenging assays. The results support the hypothesis that peptides present in the non-digestible fraction of common beans (Phaseolus vulgaris L.) may exert their physiological benefits independent of their antioxidant capacity, by ACE inhibition through interaction with its catalytic cavity.
Starting Page	1470
Ending Page	1479
Page Count	10
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	6
Issue Number	5
Journal	Food & Function
DOI	10.1039/c5fo00190k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	NDF FRAP Ferric LSGNK Enzyme Antioxidant Phaseolus vulgaris Colorectal cancer IC50 Docking (molecular) Gibbs free energy Fluorescence recovery after photobleaching DPPH Trolox Peptide ABTS Phaseolus
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

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