NDLI: Impact of soyasaponin I on TLR2 and TLR4 induced inflammation in the MUTZ-3-cell model

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Impact of soyasaponin I on TLR2 and TLR4 induced inflammation in the MUTZ-3-cell model

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Back cover

Food & Function Vol - 6 Issue - 2

Food & Function Vol -5

Food & Function Vol -4

Food & Function Vol -3

Food & Function Vol -2

Food & Function Vol -1

Impact of soyasaponin I on TLR2 and TLR4 induced inflammation in the MUTZ-3-cell model

Content Provider	Royal Society of Chemistry (RSC)
Author	Schubert, Ralf Schneider, Petra Zielen, Stefan Beermann, Christopher Fußbroich, Daniela
Copyright Year	2015
Abstract	Previous studies have demonstrated that soyasaponin (SoSa) possesses anti-inflammatory properties in lipopolysaccharide (LPS)-stimulated immune cells by influencing the immune sensing of toll-like receptor (TLR) 4. The aim of this study was to investigate the immune modulatory effect of SoSa I on TLR2- and TLR4-induced inflammation within the monocytic MUTZ-3-cell model. MUTZ-3 cells were stimulated with gram-negative (Escherichia coli) or gram-positive (Staphylococcus aureus) bacteria or bacterial pathogen-associated molecular patterns (PAMPs) such as LPS or peptidoglycans (PGN) alone or in combination with SoSa I. Cell morphology was characterized by raster scanning and light microscopy. Cytokine production (IL-1β, IL-6, TNF-α, IP-10, RANTES and IL-8) was measured by cytometric bead array and the expression of surface markers was assessed by flow cytometry. MUTZ-3 cells revealed a cell maturation-like alteration in morphology and increased expression of CD80, CD86, TLR2 and TLR4 after stimulation with either gram-negative and gram-positive bacteria or bacterial PAMPs. The addition of SoSa I suppressed pro-inflammatory cytokine and chemokine secretions in a dose-dependent manner regardless of TLR2 or TLR4 stimulation. Interestingly, E. coli- and S. aureus-induced inflammation was always inhibited better by SoSa I than that induced by LPS and PGN. Additionally, SoSa I reduced the expression of CD86 in PGN- or LPS-stimulated cells. This study demonstrated that the anti-inflammatory capacity of SoSa I is based on influencing both monocytic TLR2 and TLR4 and that SoSa I inhibits more effectively whole bacteria compared to solely LPS or PGN what points to a broader role of SoSa I in the down-regulation of inflammation.
Starting Page	1001
Ending Page	1010
Page Count	10
File Format	HTM / HTML PDF
ISSN	20426496
Volume Number	6
Issue Number	3
Journal	Food & Function
DOI	10.1039/c4fo01065e
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	TLR SoSa Lipopolysaccharide Toll-like receptor TLR2 TLR4 Inflammation Gram-negative bacteria Escherichia coli Gram-positive bacteria Aureus Bacteria Raster scan Microscopy Cytokine Interleukin 1 beta Interleukin 6 Tumor necrosis factor alpha CCL5 Interleukin 8 Bead Flow cytometry CD80 CD86 Chemokine
Content Type	Text
Resource Type	Article
Subject	Medicine Food Science

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