Lipopolysaccharide- and Lipoteichoic Acid-mediated Pro-inflammatory Cytokine Production and Modulation of TLR2, TLR4 and MyD88 Expression in Human Endometrial Cells.

Content Provider	Europe PMC
Author	Rashidi, Nesa Mirahmadian, Mahroo Jeddi-Tehrani, Mahmood Rezania, Simin Ghasemi, Jamileh Kazemnejad, Somaieh Mirzadegan, Ebrahim Vafaei, Sedigheh Kashanian, Maryam Rasoulzadeh, Zahra Zarnani, Amir-Hassan
Copyright Year	2015
Abstract	BackgroundToll-like receptor (TLR)-mediated inflammatory processes are supposed to be involved in pathophysiology of spontaneous abortion and preterm labor. Here, we investigated functional responses of human endometrial stromal cells (ESCs) and whole endometrial cells (WECs) to lipopolysaccharide (LPS) and lipoteichoic acid (LTA).MethodsEndometrial tissues were obtained from 15 cycling women who underwent laparoscopic tubal ligation. Modulation of TLR2, TLR4 and MyD88 expression and production of pro-inflammatory cytokines by WECs and ESCs in response to LPS and LTA were assessed.ResultsWECs and ESCs expressed significant levels of TLR4 and MyD88 transcripts but, unlike WECs, ESCs failed to express TLR2 gene. Regardless of positive results of Western blotting, ESCs did not express TLR4 at their surface as judged by flow cytometry. Immunofluorescent staining revealed intracellular localization of TLR4 with predominant perinuclear pattern. LPS stimulation marginally increased TLR4 gene expression in both cell types, whereas such treatment significantly upregulated MyD88 gene expression after 8 hr (p < 0.05). At the protein level, however, LPS activation significantly increased TLR4 expression by ESCs (p < 0.05). LTA stimulation of WECs was accompanied with non-significant increase of TLR2 and MyD88 transcripts. LPS and LTA stimulation of WECs caused significant production of IL-6 and IL-8 in a dose-dependent manner (p < 0.05). Similarly, ESCs produced significant amounts of IL-6, IL-8 and also TNF-α in response to LPS activation (p < 0.05).ConclusionOur results provided further evidence of initiation of inflammatory processes following endometrial TLR activation by bacterial components which could potentially be harmful to developing fetus.
Related Links	https://europepmc.org/backend/ptpmcrender.fcgi?accid=PMC4386089&blobtype=pdf
ISSN	22285482
Journal	Journal of Reproduction & Infertility [J Reprod Infertil]
Volume Number	16
PubMed Central reference number	PMC4386089
Issue Number	2
PubMed reference number	25927023
e-ISSN	2251676X
Language	English
Publisher	Avicenna Research Institute
Publisher Date	2015-04-01
Access Restriction	Open
Rights License	This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly. Copyright © 2015 Avicenna Research Institute
Subject Keyword	Cytokine Endometrium Inflammation LPS LTA Toll-like receptor
Content Type	Text
Resource Type	Article
Subject	Reproductive Medicine