NDLI: Mechanism of resonant electron emission from the deprotonated GFP chromophore and its biomimetics

Content Provider	Royal Society of Chemistry (RSC)
Author	Fielding, Helen H. Lewin, Ross Andersen, Lars H. Parkes, Michael A. Woodhouse, Joanne L. Zhang, Lijuan Mooney, Ciarán R. S. Ward, John M. Bochenkova, Anastasia V. Hailes, Helen C.
Copyright Year	2017
Abstract	The Green Fluorescent Protein (GFP), which is widely used in bioimaging, is known to undergo light-induced redox transformations. Electron transfer is thought to occur resonantly through excited states of its chromophore; however, a detailed understanding of the electron gateway states of the chromophore is still missing. Here, we use photoelectron spectroscopy and high-level quantum chemistry calculations to show that following UV excitation, the ultrafast electron dynamics in the chromophore anion proceeds via an excited shape resonance strongly coupled to the open continuum. The impact of this state is found across the entire 355–315 nm excitation range, from above the first bound–bound transition to below the opening of higher-lying continua. By disentangling the electron dynamics in the photodetachment channels, we provide an important reference for the adiabatic position of the electron gateway state, which is located at 348 nm, and discover the source of the curiously large widths of the photoelectron spectra that have been reported in the literature. By introducing chemical modifications to the GFP chromophore, we show that the detachment threshold and the position of the gateway state, and hence the underlying excited-state dynamics, can be changed systematically. This enables a fine tuning of the intrinsic electron emission properties of the GFP chromophore and has significant implications for its function, suggesting that the biomimetic GFP chromophores are more stable to photooxidation.
Starting Page	3154
Ending Page	3163
Page Count	10
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	8
Issue Number	4
Journal	Chemical Science
DOI	10.1039/c6sc05529j
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	GFP Quantum chemistry Biomimetics Green fluorescent protein Adiabatic theorem Green Fluorescent Protein Redox Ion Resonance Photoemission spectroscopy Chromophore
Content Type	Text
Resource Type	Article
Subject	Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

ortho and para chromophores of green fluorescent protein: controlling electron emission and internal conversion

sonant electron emission from the deprotonated GFP chromophore and its biomimetics †

Taking the green fluorescence out of the protein: dynamics of the isolated GFP chromophore anion

Charge transfer in green fluorescent protein

Emission enhancement and application of synthetic green fluorescent protein chromophore analogs

Electronic spectroscopy and solvatochromism in the chromophore of GFP and the Y66F mutant

Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching

Photoacid behaviour in a fluorinated green fluorescent protein chromophore: ultrafast formation of anion and zwitterion states

Understanding Optical Behaviour of Green Fluorescent Protein through It's Chromophore Analogues

Mechanism of resonant electron emission from the deprotonated GFP chromophore and its biomimetics

Similar Documents

ortho and para chromophores of green fluorescent protein: controlling electron emission and internal conversion

sonant electron emission from the deprotonated GFP chromophore and its biomimetics †

Taking the green fluorescence out of the protein: dynamics of the isolated GFP chromophore anion

Charge transfer in green fluorescent protein

Emission enhancement and application of synthetic green fluorescent protein chromophore analogs

Electronic spectroscopy and solvatochromism in the chromophore of GFP and the Y66F mutant

Photo-induced protonation/deprotonation in the GFP-like fluorescent protein Dronpa: mechanism responsible for the reversible photoswitching

Photoacid behaviour in a fluorinated green fluorescent protein chromophore: ultrafast formation of anion and zwitterion states

Understanding Optical Behaviour of Green Fluorescent Protein through It's Chromophore Analogues

Mechanism of resonant electron emission from the deprotonated GFP chromophore and its biomimetics