NDLI: Light-up probe based on AIEgens: dual signal turn-on for caspase cascade activation monitoring

Content Provider	Royal Society of Chemistry (RSC)
Author	Liu, Bin Mao, Duo Zhang, Chong-Jing Yuan, Youyong Kwok, Ryan T. K. Tang, Ben Zhong
Copyright Year	2017
Abstract	Direct monitoring of multiple enzyme activities in a given biological process is extremely important for disease diagnosis. Herein, we report a single fluorescent probe that targets two caspase activities in living cells. The probe consists of three parts that includes two AIE fluorogens with distinctive green and red emission colors excitable at a single wavelength, and a hydrophilic peptide as the substrate of the apoptosis initiator caspase-8 and the effector caspase-3. The probe is non-fluorescent in aqueous media. The green and red fluorescence can be sequentially turned on when the peptide substrate is cleaved by the cascade activation of caspase-8 and caspase-3 in early apoptotic HeLa cells induced by hydrogen peroxide. This sequential fluorescence turn-on allows real-time monitoring of the caspase cascade activation during the apoptotic process, which was further explored for evaluating the therapeutic efficiency of anticancer drugs. The probe design strategy developed in this study also proved to be general, which opens a new avenue for real-time, multiplexed imaging of cellular enzyme activity in a biological process.
Starting Page	2723
Ending Page	2728
Page Count	6
File Format	HTM / HTML PDF
ISSN	20416520
Volume Number	8
Issue Number	4
Journal	Chemical Science
DOI	10.1039/c6sc04322d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Subscribed
Subject Keyword	HeLa Hydrogen peroxide Enzyme Multiplexer Caspase Fluorescence Hydrophile Peptide Wavelength Apoptosis
Content Type	Text
Resource Type	Article
Subject	Chemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Light-Up Probe based on AIEgens : Dual Signal Turn-On for Cascade Caspase Activation Monitoring

A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection

A new label-free and turn-on fluorescence probe for hydrogen peroxide and glucose detection based on DNA–silver nanoclusters

Salidroside inhibits H2O2-induced apoptosis in PC12 cells by preventing cytochrome C release and inactivating of caspase cascade (2008)

Investigation of a halloysite-based fluorescence probe with a highly selective and sensitive “turn-on” response upon hydrogen peroxide

Colorimetric and Fluorescence Dual-Mode Biosensors Based on Peroxidase-Like Activity of the Co3O4 Nanosheets

Mercuric-triggered hydrogen peroxide “turn-on” fluorescence detection in neuronal cells with novel fluorescein-based probe obtained in one pot

A fluorescence turn-on Hg2+ probe based on rhodamine with excellent sensitivity and selectivity in living cells

Simultaneous fluorescence imaging of hydrogen peroxide in mitochondria and endoplasmic reticulum during apoptosis

Light-up probe based on AIEgens: dual signal turn-on for caspase cascade activation monitoring

Similar Documents

Light-Up Probe based on AIEgens : Dual Signal Turn-On for Cascade Caspase Activation Monitoring

A FRET probe with AIEgen as the energy quencher: dual signal turn-on for self-validated caspase detection

A new label-free and turn-on fluorescence probe for hydrogen peroxide and glucose detection based on DNA–silver nanoclusters

Salidroside inhibits H2O2-induced apoptosis in PC12 cells by preventing cytochrome C release and inactivating of caspase cascade (2008)

Investigation of a halloysite-based fluorescence probe with a highly selective and sensitive “turn-on” response upon hydrogen peroxide

Colorimetric and Fluorescence Dual-Mode Biosensors Based on Peroxidase-Like Activity of the Co3O4 Nanosheets

Mercuric-triggered hydrogen peroxide “turn-on” fluorescence detection in neuronal cells with novel fluorescein-based probe obtained in one pot

A fluorescence turn-on Hg2+ probe based on rhodamine with excellent sensitivity and selectivity in living cells

Simultaneous fluorescence imaging of hydrogen peroxide in mitochondria and endoplasmic reticulum during apoptosis

Light-up probe based on AIEgens: dual signal turn-on for caspase cascade activation monitoring