NDLI: Formation and harvesting of thick pancreatic β-cell sheets on a highly O<sub>2</sub>-permeable plate modified with poly(<i>N</i>-isopropylacrylamide)

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O²-Protected diazeniumdiolate-modified silica nanoparticles for extended nitric oxide release from dental composites

Synthetic hydrogel platform for three-dimensional culture of embryonic stem cell-derived motor neurons

Bioinspired phosphorylcholine containing polymer films with silver nanoparticles combining antifouling and antibacterial properties

Ice recrystallisation inhibition by polyols: comparison of molecular and macromolecular inhibitors and role of hydrophobic units

One-step fabrication of core–shell structured alginate–PLGA/PLLA microparticles as a novel drug delivery system for water soluble drugs

Fibroblasts remodeling of type IV collagen at a biomaterials interface

Microwave-assisted rapid synthesis of poly(glycerol-sebacate) elastomers

Formation and harvesting of thick pancreatic β-cell sheets on a highly O₂-permeable plate modified with poly(N-isopropylacrylamide)

Viscoelastic hydrogels from poly(vinyl alcohol)–Fe(iii) complex

Study on effective extraction of chicken feather keratins and their films for controlling drug release

Concentration dependent neural differentiation and neurite extension of mouse ESC on primary amine-derivatized surfaces

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Biomaterials Science Vol - 1 Issue - 4

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Formation and harvesting of thick pancreatic β-cell sheets on a highly O₂-permeable plate modified with poly(N-isopropylacrylamide)

Content Provider	Royal Society of Chemistry (RSC)
Author	Komori, Kikuo Shinohara, Marie Tsuru, Tatsuro Udagawa, Mari Sakai, Yasuyuki Montagne, Kevin
Copyright Year	2013
Abstract	Producing sheet-like tissues is a promising strategy for implantable engineered tissues, because in vitro pre-vascularization is dispensable in this configuration. We developed a simple methodology for the formation and non-destructive harvesting of a thick pancreatic β-cell sheet consisting of mouse insulinoma MIN6-m9 cells and mouse NIH3T3 fibroblasts using an O2-permeable polydimethylsiloxane plate modified with poly(N-isopropylacrylamide) (O2+/PNIPA–PDMS plate). Owing to the direct oxygenation of the cells through the PNIPA-modified PDMS plate, a viable, metabolically active sheet 5–6 cell layers thick (ca. 60 μm thick) was formed spontaneously; in the absence of direct oxygenation, only a thin cell sheet could be formed consisting of at most 2 layers (ca. 20 μm thick) with mainly anaerobic metabolism. Consequently, the net density of MIN6-m9 cells under direct oxygenation was about twice as high as in the absence of direct oxygenation. Accordingly, the insulin secretion for 10 to 60 min after glucose stimulation was also about 1.5 times higher with oxygenation. Furthermore, the thick cell sheet was successfully harvested from the O2+/PNIPA–PDMS plate surface in a non-destructive manner by inducing a phase transition of PNIPA by lowering the temperature below the lower critical solution temperature. Thus, the present report shows a promising and simple method to produce thick sheet-like engineered tissues for transplantation that could be used as a treatment for type 1 diabetes.
Starting Page	510
Ending Page	518
Page Count	9
File Format	HTM / HTML PDF
ISSN	20474830
Volume Number	1
Issue Number	5
Journal	Biomaterials Science
DOI	10.1039/c3bm00123g
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Oxygen Mouse Poly(N-isopropylacrylamide) Insulinoma Beta cell Micrometre Glucose Fermentation Polydimethylsiloxane Insulin Diabetes mellitus type 1 Phase transition
Content Type	Text
Resource Type	Article
Subject	Materials Science Biomedical Engineering

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Controlled Release of Insulin Based on Temperature and Glucose Dual Responsive Biomicrocapsules

Insights into pancreatic β cell energy metabolism using rodent β cell models

Prolactin induction of insulin gene expression: the roles of glucose and glucose transporter-2.

GABA signalling in human pancreatic islets

Effects of Micro-Features on Cell Detachment from Poly ( N-isopropylacrylamide ) Coated Polydimethylsiloxane Membranes

TCF7L2 in mouse pancreatic beta cells plays a crucial role in glucose homeostasis by regulating beta cell mass

Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression

miR-184 Regulates Pancreatic β-Cell Function According to Glucose Metabolism

Pancreatic beta cell lines and their applications in diabetes mellitus research.

Formation and harvesting of thick pancreatic β-cell sheets on a highly O₂-permeable plate modified with poly(N-isopropylacrylamide)

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Formation and harvesting of thick pancreatic β-cell sheets on a highly O₂-permeable plate modified with poly(N-isopropylacrylamide)