NDLI: A camelid VHH-based fluorescence polarization immunoassay for the detection of tetrabromobisphenol A in water

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A camelid VHH-based fluorescence polarization immunoassay for the detection of tetrabromobisphenol A in water

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Analytical Methods Vol - 8 Issue - 29

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A camelid VHH-based fluorescence polarization immunoassay for the detection of tetrabromobisphenol A in water

Content Provider	Royal Society of Chemistry (RSC)
Author	Ji, Pei Li, Qing X. Eremin, Sergei A. Liu, Zhiping Wang, Kai Li, Ji Liu, Jianfeng Xu, Ting
Copyright Year	2016
Abstract	Tetrabromobisphenol A (TBBPA) is a widely used flame retardant and is classified as an endocrine disruptor. A convenient and sensitive fluorescence polarization immunoassay (FPIA) for TBBPA was developed with a camelid variable domain of heavy chain antibody (VHH) being specific for TBBPA. 3-(2,6-Dibromo-4-(2-(3,5-dibromo-4-hydroxyphenyl)propan-2-yl))propanoic acid (hapten T1) was conjugated with ethylenediamine fluorescein thiocarbamoyl (EDF) to form a tracer that binds with anti-TBBPA VHH. The limit of detection and half-maximum inhibition concentration of TBBPA by FPIA were 5 ng mL−1 and 90 ng mL−1, respectively. This FPIA was used to detect TBBPA in water samples without any pre-treatment and each analysis was performed within 20–30 min. The recovery of TBBPA from spiked water samples ranged from 73% to 102%, with coefficients of variation ranging from 7% to 14%. The FPIA exhibited high potential for rapid and accurate determination of TBBPA in real water samples.
Starting Page	7265
Ending Page	7271
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	8
Issue Number	39
Journal	Analytical Methods
DOI	10.1039/c6ay01603k
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Acid FPIA EDF Camelid Flame retardant Endocrine disruptor Hapten Ethylenediamine Tetrabromobisphenol A Fluorescein Fluorescence polarization immunoassay European Development Fund TBBPA
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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A camelid VHH-based fluorescence polarization immunoassay for the detection of tetrabromobisphenol A in water

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