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A Sensitive Fluorescence Polarization Immunoassay for the Rapid Detection of Okadaic Acid in Environmental Waters.
| Content Provider | Europe PMC |
|---|---|
| Author | Hendrickson, Olga D. Mukhametova, Liliya I. Zvereva, Elena A. Zherdev, Anatoly V. Eremin, Sergei A. |
| Copyright Year | 2023 |
| Abstract | In this study, a homogeneous fluorescence polarization immunoassay (FPIA) for the detection of hazardous aquatic toxin okadaic acid (OA) contaminating environmental waters was for the first time developed. A conjugate of the analyte with a fluorophore based on a fluorescein derivative (tracer) was synthesized, and its interaction with specific anti-OA monoclonal antibodies (MAbs) was tested. A MAbs–tracer pair demonstrated highly affine immune binding (KD = 0.8 nM). Under optimal conditions, the limit of OA detection in the FPIA was 0.08 ng/mL (0.1 nM), and the working range of detectable concentrations was 0.4–72.5 ng/mL (0.5–90 nM). The developed FPIA was approbated for the determination of OA in real matrices: river water and seawater samples. No matrix effect of water was observed; therefore, no sample preparation was required before analysis. Due to this factor, the entire analytical procedure took less than 10 min. Using a compact portable fluorescence polarization analyzer enables the on-site testing of water samples. The developed analysis is very fast, easy to operate, and sensitive and can be extended to the determination of other aquatic toxins or low-molecular-weight water or food contaminants. |
| Volume Number | 13 |
| PubMed Central reference number | PMC10136290 |
| Issue Number | 4 |
| PubMed reference number | 37185552 |
| Journal | Biosensors (Basel) |
| e-ISSN | 20796374 |
| DOI | 10.3390/bios13040477 |
| Language | English |
| Publisher | MDPI |
| Publisher Date | 2023-04-16 |
| Access Restriction | Open |
| Rights License | Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors. |
| Subject Keyword | fluorescence polarization immunoassay phycotoxins okadaic acid contamination water safety |
| Content Type | Text |
| Resource Type | Article |
| Subject | Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology |
