NDLI: BPA Endocrine Disruptor Detection at the Cutting Edge: FPIA and ELISA Immunoassays.

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BPA Endocrine Disruptor Detection at the Cutting Edge: FPIA and ELISA Immunoassays.

Content Provider	Europe PMC
Author	Raysyan, Anna Zwigart, Sandro D. Eremin, Sergei A. Schneider, Rudolf J.
Copyright Year	2023
Abstract	BPA is a chemical commonly used in the production of polymer-based materials that can have detrimental effects on the thyroid gland and impact human reproductive health. Various expensive methods, such as liquid and gas chromatography, have been suggested for detecting BPA. The fluorescence polarization immunoassay (FPIA) is an inexpensive and efficient homogeneous mix-and-read method that allows for high-throughput screening. FPIA offers high specificity and sensitivity and can be carried out in a single phase within a timeframe of 20–30 min. In this study, new tracer molecules were designed that linked the fluorescein fluorophore with and without a spacer to the bisphenol A moiety. To assess the influence of the C6 spacer on the sensitivity of an assay based on the respective antibody, hapten–protein conjugates were synthesized and assessed for performance in an ELISA setup, and this resulted in a highly sensitive assay with a detection limit of 0.05 g/L. The lowest limit of detection was reached by employing the spacer derivate in the FPIA and was 1.0 μg/L, working range from 2 to 155 μg/L. The validation of the methods was conducted using actual samples compared to LC–MS/MS, which served as the reference method. The FPIA and ELISA both demonstrated satisfactory concordance.
Volume Number	13
PubMed Central reference number	PMC10296492
Issue Number	6
PubMed reference number	37367029
Journal	Biosensors (Basel)
e-ISSN	20796374
DOI	10.3390/bios13060664
Language	English
Publisher	MDPI
Publisher Date	2023-06-19
Access Restriction	Open
Rights License	Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). © 2023 by the authors.
Subject Keyword	FPIA ELISA immunoassay bisphenol A endocrine disruptor
Content Type	Text
Resource Type	Article
Subject	Clinical Biochemistry Instrumentation Medicine Biomedical Engineering Engineering Analytical Chemistry Biotechnology

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