NDLI: Identification and characterization of stress degradation products of piperine and profiling of a black pepper (<i>Piper nigrum</i> L.) extract using LC/Q-TOF-dual ESI-MS

Content Provider	Royal Society of Chemistry (RSC)
Author	Murali, P. M. Dubey, P. K. Kotte, Subhash Chandra Bose.
Copyright Year	2014
Abstract	A rapid, specific and reliable high-performance liquid chromatography combined with quadrupole time-of-flight dual electrospray ionization mass spectrometry (LC/Q-TOF-dual ESI-MS) method has been developed and validated for the identification and characterization of stressed degradation products of piperine. Piperine, an anti-hypertensive drug, was subjected to hydrolysis (acidic, alkaline and neutral), oxidation, photolysis and thermal stress, as per ICH-specified conditions. The drug showed extensive degradation under oxidation and hydrolysis (acid and base) stress conditions. However, it was more stable under thermal stress than under acidic, alkaline, neutral and photolysis stress conditions. A total of four degradation products were observed and the chromatographic separation of the drug and its degradation products was achieved on a C18 column (4.6 × 50 mm, 5 μm). To characterize the degradation products, fragmentation patterns and accurate masses of the degradation products were established by subjecting them to LC-MS/Q-TOF analysis. Structure elucidation of the degradation products was achieved by comparing their fragmentation patterns with that of the drug, and confirmation was achieved through profiling a black pepper extract (Piper nigrum L.). The method identified dihydropiperine, piperylin, piperlonguminine, trans-piperine, cis-piperine, dihydropiperlonguminine, trans-piperettine and cis-piperettine. The liquid chromatography-mass spectroscopy method was validated with respect to its specificity, linearity, accuracy and precision.
Starting Page	8022
Ending Page	8029
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	19
Journal	Analytical Methods
DOI	10.1039/c4ay01088d
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Spectroscopy Acid Electrospray ionization Photodissociation High-performance liquid chromatography Time-of-flight mass spectrometry Chromatography Quadrupole mass analyzer Hydrolysis Piperine Ionization Micrometre Black pepper Piper Mass spectrometry
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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