NDLI: A portable and quantitative enzyme immunoassay of neuron-specific enolase with a glucometer readout

Content Provider	Royal Society of Chemistry (RSC)
Author	Feng, Xueru Xu, Kun Huang, Rong Fu, Xiaohong
Copyright Year	2014
Abstract	A portable and quantitative enzyme immunoassay with a glucometer readout was developed for the sensitive monitoring of neuron-specific enolase (NSE, as a model analyte) in a high-binding polystyrene 96-well microtiter plate (MTP), conjugated with monoclonal mouse anti-human NSE antibody (mAb1). Gold nanoparticles heavily functionalized with glucoamylase and polyclonal rabbit anti-human NSE antibody (pAb2) were utilized as the trace tag. A sandwich-type immunoassay format was adopted for the quantitative detection of NSE in the mAb1-functionalized MTP. Accompanying the gold nanoparticles, the carried glucoamylase could hydrolyze amylopectin in glucose. The produced glucose could be quantitatively monitored using a portable personal glucose meter (PGM). Under optimal conditions, the PGM-based immunoassay exhibited good analytical properties for the determination of the target NSE, and allowed the detection of NSE at concentrations as low as 0.05 ng mL−1. The intra- and inter-assay coefficients of variation (CVs) were below 10% and 11%, respectively. The methodology was also evaluated by assaying 15 clinical serum samples, and showed good accordance between results obtained by the developed immunoassay and the referenced values.
Starting Page	2233
Ending Page	2238
Page Count	6
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	7
Journal	Analytical Methods
DOI	10.1039/c3ay42075b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	NSE Enolase 2 Analyte Immunoassay Microtiter plate Amylopectin MTP Monoclonal antibody Glucan 1,4-a-glucosidase Glucose Polystyrene Mouse Polyclonal antibodies ELISA Glucose meter
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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