NDLI: Multiplexed miRNA detection using cationic polythiophene

Content Provider	Royal Society of Chemistry (RSC)
Author	Chase, Thomas E. He, Lin Zheng, Weiming
Copyright Year	2014
Abstract	Detection of short strands of miRNA has significant biomedical and biological implications. We report here the integration of fluorescent conjugated polymers as detection moieties with metallic striped nanorods for multiplexed detection of miR-21 and miR-122 in solution. Specifically, cationic conjugated polymers were used as chemical-coupling-free signal reporters to indicate the presence of specific miRNA molecules in solution and nanorods with self-encoded striping patterns were used as multiplexing assay substrates. A competitive assay format was developed to take advantage of stronger fluorescence output from DNA/DNA duplexes than that from DNA/miRNA duplexes. Upon selection of optimal competing DNA probes, the fluorescence signal collected was inversely proportional to the concentration of target miRNA in the system. A detection limit of fmol amounts of miRNA was achieved, with a linear response spanning across a broad dynamic range. The use of inherently encoded nanorods allowed simultaneous detection of different miRNA targets with good specificity.
Starting Page	2399
Ending Page	2405
Page Count	7
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	7
Journal	Analytical Methods
DOI	10.1039/c3ay41752b
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Multiplexing DNA Fluorescence Dynamic range
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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