NDLI: Parallel assay of inkjet-printed cytochrome P450

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Back cover

Analytical Methods Vol - 6 Issue - 6

Analytical Methods Vol - 6 Issue - 5

Analytical Methods Vol - 6 Issue - 4

Analytical Methods Vol - 6 Issue - 3

Analytical Methods Vol -5

Analytical Methods Vol -4

Analytical Methods Vol -3

Analytical Methods Vol -2

Analytical Methods Vol -1

Parallel assay of inkjet-printed cytochrome P450

Content Provider	Royal Society of Chemistry (RSC)
Author	Tatsu, Yoshiro Goto, Tatsushi Kanemura, Emi Morigaki, Kenichi Imaishi, Hiromasa
Copyright Year	2014
Abstract	Assaying the activities of cytochrome P450 (P450) is important for evaluating the toxicity of chemicals in drugs, food, and the environment. We developed a methodology to immobilize multiple P450 isoforms with a heightened density and assay their activities in parallel. Inkjet printing technology was applied for the deposition of P450 onto a plastic substrate (cycloolefin polymer: COP) having arrayed microwells (diameter: 50 μm, depth: 60 μm). P450 was printed into each microwell and immobilized by gelation of co-printed agarose. Activities of printed P450 isoforms were measured by introducing a substrate solution into the microwells and sealing it with an elastomer sheet, isolating individual wells. In order to avoid uncontrolled initiation of the reaction, we photo-regulated the P450 catalysis by using caged glucose-6-phosphate. The combination of inkjet printing, immobilization in microwells, and photo-regulated initiation of the enzymatic reaction enabled us to assay multiple P450 isoforms on a chip with an unprecedented density. We demonstrate the parallel assay of single nucleotide polymorphs of P450, which play important roles in different drug efficacies in individuals.
Starting Page	2117
Ending Page	2124
Page Count	8
File Format	HTM / HTML PDF
ISSN	17599660
Volume Number	6
Issue Number	7
Journal	Analytical Methods
DOI	10.1039/c3ay41446a
Language	English
Publisher	Royal Society of Chemistry
Access Restriction	Open
Subject Keyword	Glucose 6-phosphate Cytochrome P450 Micrometre Elastomer Granulocyte Nucleotide Inkjet printing Polymer Agarose
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Engineering Chemical Engineering

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